Abstract |
We have already demonstrated that inactivated, replication-defective Sendai virus particles (HVJ-E) have a powerful antitumor effect by both the generation of tumor-specific cytotoxic T cells and inhibition of regulatory T cell activity. Here, we report that HVJ-E also has an antitumor effect through non-T cell immunity. Microarray analysis revealed that direct injection of HVJ-E induced the expression of CXCL10 in established Renca tumors. CXCL10 was secreted by dendritic cells in the tumors after HVJ-E injection. Quantitative real-time RT-PCR and immunohistochemistry revealed that CXCR3+ cells (predominantly NK cells) infiltrated the HVJ-E-injected tumors. Moreover, HVJ-E injection caused systemic activation of NK cells and enhanced their cytotoxity against tumor cells. In an in vivo experiment, approximately 50% of tumors were eradicated by HVJ-E injection, and this activity of HVJ-E against Renca tumors was largely abolished by NK cell depletion using anti- asialo GM1 antibody. Since HVJ-E injection induced systemic antitumor immunity by enhancing or correcting the chemokine- chemokine receptor axis, it might be a potential new therapy for cancer.
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Authors | Atsuko Fujihara, Masayuki Kurooka, Tsuneharu Miki, Yasufumi Kaneda |
Journal | Cancer immunology, immunotherapy : CII
(Cancer Immunol Immunother)
Vol. 57
Issue 1
Pg. 73-84
(Jan 2008)
ISSN: 0340-7004 [Print] Germany |
PMID | 17602226
(Publication Type: Journal Article)
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Chemical References |
- Chemokine CXCL10
- RNA, Messenger
|
Topics |
- Animals
- Chemokine CXCL10
(biosynthesis)
- Female
- Fluorescent Antibody Technique
- Genes, Viral
- Immunohistochemistry
- Immunotherapy
(methods)
- Killer Cells, Natural
(immunology)
- Lymphocyte Activation
(immunology)
- Mice
- Mice, Inbred BALB C
- Neoplasms, Experimental
(immunology, therapy)
- Oligonucleotide Array Sequence Analysis
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Sendai virus
(immunology)
- Virion
(immunology)
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