Abstract |
Allogeneic mixed lymphocyte tumor cell cultures (MLTCs) were established using lymphocytes from non-small-cell lung cancer (NSCLC) patient UKY-53 and HLA-A2+ NSCLC tumor cells (UKY-29). The tumor cells expressed the lymphocyte costimulatory molecule CD80 (UKY29.7). Cytolytic activity showed the cytotoxic T-lymphocytes (CTL) lysed UKY-29, but not K562 or Daudi. The CTL also lysed: HLA-A2+ and -A24+ tumor cell lines from a number of tumor histologies. The CTL also lysed Epstein Barr virus transformed (EBV) B-cells, UKY-29EBV, autologous to the stimulating cell line, UKY29TC. These data suggested the presence of both tumor-specific and allogeneic reactivities in the bulk CTL population. Subsequent cDNA cloning analysis and sequencing demonstrated that the bulk CTL population was recognizing: (i) allogeneic target HLA-CW3, and two minor histocompatibility antigens; (ii) guanine nucleotide- binding protein, G(S) (GNAS), and (iii) inositol myophosphatase ( IMPA). All three antigens, we believe, were restricted by HLA-A2. Whereas the system described was initially intended to identify tumor-associated antigens recognized by CTL, the nature of the allogeneic system provides a unique opportunity for the identification of epitopes that confer both allo and minor antigen recognition.
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Authors | Joanne M Wroblewski, John R Yannelli |
Journal | Cancer biotherapy & radiopharmaceuticals
(Cancer Biother Radiopharm)
Vol. 22
Issue 2
Pg. 206-22
(Apr 2007)
ISSN: 1084-9785 [Print] United States |
PMID | 17600468
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Chromogranins
- DNA, Complementary
- HLA-A2 Antigen
- HLA-C Antigens
- HLA-C*03 antigen
- RNA, Messenger
- Phosphoric Monoester Hydrolases
- GNAS protein, human
- GTP-Binding Proteins
- GTP-Binding Protein alpha Subunits, Gs
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Topics |
- Base Sequence
- Cell Line, Tumor
- Chromogranins
- DNA, Complementary
(genetics)
- GTP-Binding Protein alpha Subunits, Gs
(genetics, immunology)
- GTP-Binding Proteins
(genetics, immunology)
- Gene Library
- HLA-A2 Antigen
(immunology)
- HLA-C Antigens
(genetics, immunology)
- Humans
- Lymphocyte Culture Test, Mixed
- Molecular Sequence Data
- Phosphoric Monoester Hydrolases
(genetics, immunology)
- RNA, Messenger
(genetics)
- Sensitivity and Specificity
- T-Lymphocytes, Cytotoxic
(immunology, metabolism)
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