Abstract | BACKGROUND & OBJECTIVE: The herbal formulation, Gamiseunggal-Tang (G-Tang) has long been used for various allergic diseases. The mechanism of its action is largely unknown. We carried out this study to determine the effect of G-Tang on the mast cell-mediated anaphylactic reactions in vivo and in vitro murine models. METHODS: In this study, the effects of G-Tang on the mast cell-mediated anaphylactic reactions were examined by using the ear swelling, histamine assay, and ELISA method in murine model. RESULTS: Anal administration of G-Tang showed dose-dependent inhibitory activity on the compound 48/80-induced ear swelling response (P<0.05) and histamine release (P<0.01). G-Tang (0.001-0.1 g/kg) significantly inhibited passive cutaneous anaphylaxis (P<0.05) in mice. The production of tumour necrosis factor-alpha ( TNF-alpha) was also significantly inhibited (about 47.4%, at 0.1 mg/ml, P<0.01) by treatment of G-tang in anti-dinitrophenyl IgE antibodystimulated mast cells. INTERPRETATION & CONCLUSION: Findings of our study showed that G-Tang inhibited immediate type allergic reaction in a murine model and may be beneficial in the treatment of allergic inflammatory diseases.
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Authors | Hyun-Ja Jeong, Phil-Dong Moon, Jae-Young Um, Jinhan Park, Kang-Hyun Leem, Chang-Ju Kim, Hyung-Min Kim, Seung-Heon Hong |
Journal | The Indian journal of medical research
(Indian J Med Res)
Vol. 125
Issue 4
Pg. 542-9
(Apr 2007)
ISSN: 0971-5916 [Print] India |
PMID | 17598940
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Dinitrophenols
- Plant Extracts
- Tumor Necrosis Factor-alpha
- Immunoglobulin E
- p-Methoxy-N-methylphenethylamine
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Topics |
- Anaphylaxis
(chemically induced, immunology)
- Animals
- Cells, Cultured
- Dinitrophenols
(immunology)
- Ear
(anatomy & histology)
- Histamine Release
(drug effects)
- Humans
- Immunoglobulin E
(immunology)
- Korea
- Mast Cells
(drug effects, immunology)
- Medicine, East Asian Traditional
- Mice
- Plant Extracts
(immunology, pharmacology)
- Rats
- Rats, Wistar
- Tumor Necrosis Factor-alpha
(immunology)
- p-Methoxy-N-methylphenethylamine
(immunology, pharmacology)
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