| Abstract | Our previous study has shown the alteration of C cells activity in rats with experimental model of hyperthyroidism. The aim of the present study was the evaluation of parafollicular cells activity in rats with hypothyroidism evoked by propylthiouracil (PTU) given in drinking water over 21 days. Histological, ultrastructural and immunocytochemical studies using specific antibodies against calcitonin and CGRP were performed on thyroid glands taken from experimental and control groups of rats. Moreover, in all animals the calcitonin plasma levels were evaluated by radioimmunoassay. After chronic administration of PTU, thyroid image showed predominant microfollicular hyperplasia and attenuated density of parafollicular cells. The intensity of immunocytochemical reactions for CT and CGRP were weaker in the majority of C cells in comparison to the control rats, in which strong immunocytochemical reaction was observed. Examination in the electron microscope reveals the features of hypoactivity both in follicular and parafollicular cells, in which the quantity and electron density of secretory granules were smaller in comparison to the control group. These microscopic changes were accompanied by a significant decrease of calcitonin plasma concentration. Alteration of C cells activity in the experimental model of hypothyroidism, accompanied by microfollicular hypertrophy, may point to the mutual cooperation between parafollicular and follicular cells. |
| Authors | Robert Lukasz Zbucki, Maria Malgorzata Winnicka, Boguslaw Sawicki, Beata Szynaka, Anna Andrzejewska, Zbigniew Puchalski
(Affiliation: Department of General and Experimental Pathology, Medical University of Białystok, Poland. rzbucki at wp.pl)
|
| Journal | Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society
(Folia Histochem Cytobiol)
Vol. 45
Issue 2
Pg. 115-21
( 2007)
ISSN: 0239-8508 Poland |
| PMID | 17597025
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Propylthiouracil
- Calcitonin Gene-Related Peptide
- Calcitonin
|
| Topics |
- Animals
- Calcitonin
(blood)
- Calcitonin Gene-Related Peptide
(metabolism)
- Hypothyroidism
(chemically induced, pathology)
- Male
- Propylthiouracil
(pharmacology)
- Radioimmunoassay
- Rats
- Rats, Wistar
- Thyroid Gland
(cytology, pathology, ultrastructure)
|
