IL-10 measurement in aqueous humor for screening patients with suspicion of primary intraocular lymphoma.

Abstract

PURPOSE:

To determine the value of IL-10 measurement in aqueous humor (AH) for screening in primary intraocular lymphoma (PIOL).

METHODS:

One hundred consecutive diagnostic or therapeutic vitrectomies were performed in patients with uveitis. During surgery, 100 microL of both AH and pure vitreous was taken. IL-10 levels were determined with a standard quantitative sandwich enzyme immunoassay technique. Patients were distributed in two groups: 51 patients with proven PIOL, 108 patients with uveitis divided into 74 with uveitis of proven etiology and 34 with idiopathic uveitis. Groups were compared by ANOVA and the Tukey-Kramer test or nonparametric Wilcoxon test. Distributions were compared by using the chi(2) test. Segmentation was derived from the ROC curves by choosing a tradeoff between sensitivity and specificity.

RESULTS:

In patients with PIOL, IL-10 mean values were 2205.5 pg/mL (median: 1467 pg/mL) in the vitreous and 543.4 pg/mL (median: 424 pg/mL) in AH. In patients with uveitis (idiopathic and diagnostic uveitis), mean values were 26.6 pg/mL (median: 8 pg/mL) in the vitreous, and 21.9 pg/mL (median: 8 pg/mL) in AH. IL-10 mean values were significantly different between patients with PIOL and patients with uveitis (P < 10(-3)). The areas under the curves were 0.989 and 0.962 for vitreous and AH, respectively. A cutoff of 50 pg/mL in the AH was associated with a sensitivity of 0.89 and a specificity of 0.93. In the vitreous, a cutoff value of 400 pg/mL yielded a specificity of 0.99 and a sensitivity of 0.8.

CONCLUSIONS:

Diagnosis of PIOL is often made months or years after the initial onset of ocular symptoms. Cytology remains the gold standard for diagnosis. However, measurement of IL-10 in the AH is a good screening test to reduce diagnostic delays.

• Authors: Nathalie Cassoux, Alain Giron, Bahram Bodaghi, Thi H C Tran, Sylvie Baudet, Fréderic Davy, Chi C Chan, Phuc Lehoang, Hélène Merle-Béral
• Journal: Investigative ophthalmology & visual science (Invest Ophthalmol Vis Sci) Vol. 48 Issue 7 Pg. 3253-9 (Jul 2007) ISSN: 0146-0404 [Print] United States
• PMID: 17591896 (Publication Type: Journal Article)

Chemical References

• IL10 protein, human
• Interleukin-10

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Aqueous Humor (metabolism)
• Child
• Eye Neoplasms (diagnosis, metabolism)
• Female
• Humans
• Immunoenzyme Techniques
• Interleukin-10 (metabolism)
• Lymphoma, B-Cell (diagnosis, metabolism)
• Lymphoma, Large B-Cell, Diffuse (diagnosis, metabolism)
• Male
• Middle Aged
• ROC Curve
• Sensitivity and Specificity
• Uveitis (metabolism)
• Vitrectomy
• Vitreous Body (metabolism, pathology)