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[Comparison of different methods of DNA extraction from blood to detect fungal DNA by PCR].

Abstract
Comparison of different methods of DNA extraction from blood to detect fungal DNA by PCR. Invasive fungal infections (IFI) are associated with high mortality by reaching levels of 50%, and also with a significant failure in antifungical treatments. This fact mostly obeys to difficulties in obtaining a fast and accurate mycologic diagnosis due to the low sensitivity of conventional methods, mainly in neutropenic and AIDS patients. Various methods based on fungal DNA study are currently being used for the diagnosis of mycotic infections. We herein evaluated two procedures of extraction and purification of fungal DNA in blood for their use in PCR detection. Both of them showed equal efficiency in obtaining high performance DNA with universal primers ITS land ITS 4 as target.
AuthorsV M Jewtuchowicz, J L Finquelievich, E Durán, M T Mujica, C A Iovannitti
JournalRevista Argentina de microbiologia (Rev Argent Microbiol) 2007 Jan-Mar Vol. 39 Issue 1 Pg. 15-8 ISSN: 0325-7541 [Print] Argentina
Vernacular TitleComparación de métodos de extracción de ADN de sangre para detectar ADN fúngico por PCR.
PMID17585652 (Publication Type: Comparative Study, English Abstract, Journal Article)
Chemical References
  • DNA, Fungal
Topics
  • Blood Chemical Analysis (methods)
  • DNA, Fungal (blood, isolation & purification)
  • Humans
  • Polymerase Chain Reaction

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