In vivo detection of intrinsic reactive oxygen species using acyl-protected hydroxylamine in puromycin nephrosis.

Abstract

Intrinsic reactive oxygen species (ROS) in a rat model of human minimal change nephropathy were detected directly using an in vivo electron paramagnetic resonance (EPR) method with 1-acetoxy-3-carbamoyl-2,2,5,5-tetramethylpyrrolidine (ACP) in real time. The nephrosis was induced by the intravenous administration of 75 mg/kg of puromycin aminonucleoside (PAN). It was found that ROS in the kidney were increased 1 h after the administration of PAN. This increased oxidative stress declined at 24 h and returned to a normal level 3 days after PAN administration. This is the first non-invasive in vivo detection and quantification of specific ROS in an experimental nephrosis model.

• Authors: Atsushi Ueda, Aki Hirayama, Sohji Nagase, Mariko Inoue, Takaaki Oteki, Masaaki Aoyama, Hidekatsu Yokoyama
• Journal: Free radical research (Free Radic Res) Vol. 41 Issue 7 Pg. 823-8 (Jul 2007) ISSN: 1071-5762 [Print] England
• PMID: 17577743 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Pyrrolidines
• Reactive Oxygen Species
• Hydroxylamine
• Puromycin Aminonucleoside
• Catalase
• Superoxide Dismutase
• Dimethyl Sulfoxide

Topics

• Animals
• Catalase (pharmacology)
• Dimethyl Sulfoxide (pharmacology)
• Disease Models, Animal
• Electron Spin Resonance Spectroscopy
• Hydroxylamine (pharmacology)
• Kinetics
• Nephrosis (chemically induced, metabolism, pathology)
• Puromycin Aminonucleoside (toxicity)
• Pyrrolidines (toxicity)
• Rats
• Reactive Oxygen Species (metabolism)
• Superoxide Dismutase (pharmacology)