[Effects of rotundine injection on nitric oxide synthase in tissues of different organs after ischemia/reperfusion of brain in rats].

Abstract

OBJECTIVE:

To explore the protective effect of rotundine injection on lung, liver and kidney damages after cerebral ischemia/reperfusion (I/R) injury in rats based on the activity changes of nitric oxide synthase (NOS).

METHODS:

Seventy-six rats were randomly divided into three groups: sham operation group, I/R injury group and treatment group, and determinations were done at five different time points. The cerebral I/R models were reproduced by improved 4 vessels occlusion method. The activities of NOS in the lung, liver and kidney were measured in all the rats at 2, 6, 12, 24 and 48 hours after reperfusion.

RESULTS:

Compared with sham operation group, the activities of total NOS (tNOS) were significantly increased at 2, 12 and 24 hours in I/R injury group (P<0.05 or P<0.01), with the peak value at 12 hours (all P<0.01). The activities of constitutive NOS (cNOS) were increased significantly at 2 hours (all P<0.05), and those of induced NOS (iNOS) were increased at 12 hours (all P<0.01). The activities of iNOS were still high at 24 hours (all P<0.05), and approached the levels of sham operation group at 48 hours. Compared with I/R injury group, the activities of cNOS in various organs increased much higher at 2 hours in treatment group (all P<0.05). But those of iNOS were significantly decreased after 12 hours (P<0.05 or P<0.01).

CONCLUSION:

The various types of NOS play different roles in the lung damages after brain I/R injury at different stages in rats. Rotundine injection can ameliorate the damages by modulating the activities of different types of NOS.

• Authors: Ai-ying Zeng, En-yuan Tu, Zun-yi Han, Guang-tian Yang
• Journal: Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue (Zhongguo Wei Zhong Bing Ji Jiu Yi Xue) Vol. 19 Issue 6 Pg. 361-4 (Jun 2007) ISSN: 1003-0603 [Print] China
• PMID: 17577446 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Berberine Alkaloids
• tetrahydropalmatine
• Nitric Oxide Synthase
• Nitric Oxide Synthase Type I
• Nos1 protein, rat

Topics

• Animals
• Berberine Alkaloids (pharmacology)
• Brain Ischemia (enzymology)
• Disease Models, Animal
• Female
• Kidney (enzymology)
• Liver (enzymology)
• Lung (enzymology)
• Male
• Nitric Oxide Synthase (metabolism)
• Nitric Oxide Synthase Type I
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (enzymology)