Abstract |
The marine natural product thiocoraline A displayed approximately equal cytotoxic activity at nanomolar concentrations in a panel of 12 human cancer cell lines. X-ray diffraction analyses of orthorhombic crystals of this DNA-binding drug revealed arrays of docked pairs of staple-shaped molecules in which one pendent hydroxyquinoline chromophore from each cysteine-rich molecule appears intercalated between the two chromophores of a facing molecule. This arrangement is in contrast to the proposed mode of binding to DNA that shows the two drug chromophores clamping two stacked base pairs, in agreement with the nearest-neighbor exclusion principle. Proof of DNA sequence recognition was obtained from both classical DNase I footprinting experiments and determination of the melting temperatures of several custom-designed fluorescently labeled oligonucleotides. A rationale for the DNA-binding behavior was gained when models of thiocoraline clamping a central step embedded in several octanucleotides were built and studied by means of unrestrained molecular dynamics simulations in aqueous solution.
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Authors | Ana Negri, Esther Marco, Verónica García-Hernández, Alberto Domingo, Antonio L Llamas-Saiz, Silvia Porto-Sandá, Ricardo Riguera, William Laine, Marie-Hélène David-Cordonnier, Christian Bailly, Luis F García-Fernández, Juan José Vaquero, Federico Gago |
Journal | Journal of medicinal chemistry
(J Med Chem)
Vol. 50
Issue 14
Pg. 3322-33
(Jul 12 2007)
ISSN: 0022-2623 [Print] United States |
PMID | 17571868
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents
- Depsipeptides
- thiocoraline
- DNA
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Topics |
- Antineoplastic Agents
(chemistry, metabolism, pharmacology)
- Base Sequence
- Cell Line, Tumor
- Crystallography, X-Ray
- DNA
(metabolism)
- DNA Footprinting
- Depsipeptides
(chemistry, metabolism, pharmacology)
- Humans
- Stereoisomerism
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