Abstract |
Neural stem cells (NSCs) hold great promise for glioma therapy due to their inherent tumor-tropic properties, enabling them to deliver therapeutic agents directly to invasive tumor sites. In the present study, we visualized and quantitatively analyzed the spatial distribution of tumor-tropic NSCs in a mouse model of orthotopic glioma in order to predict the therapeutic efficacy of a representative NSC-based glioma therapy. U251.eGFP human glioma was established in the brain of athymic mice, followed by stereotactic injection of CM-DiI-labeled human NSCs posterior-lateral to the tumor site. Confocal microscopy, three-dimensional modeling and mathematical algorithms were used to visualize and characterize the spatial distribution of NSCs throughout the tumor. The pattern of NSC distribution showed a gradient with higher densities toward the centroid of the tumor mass. We estimate that NSC-mediated therapy would eradicate 70-90% of the primary tumor mass and the majority of invasive tumor foci. Our method may serve as a model for optimizing the efficacy of NSC-based glioma therapy.
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Authors | David Lin, Joseph Najbauer, Paul M Salvaterra, Adam N Mamelak, Michael E Barish, Elizabeth Garcia, Marianne Z Metz, Stephen E Kendall, Marisa Bowers, Babak Kateb, Seung U Kim, Margaret Johnson, Karen S Aboody |
Journal | NeuroImage
(Neuroimage)
Vol. 37 Suppl 1
Pg. S18-26
( 2007)
ISSN: 1053-8119 [Print] United States |
PMID | 17560798
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Green Fluorescent Proteins
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Topics |
- Algorithms
- Brain Neoplasms
(genetics, pathology, therapy)
- Cell Line, Tumor
- Gene Transfer Techniques
- Glioma
(genetics, pathology, therapy)
- Green Fluorescent Proteins
- Humans
- Image Processing, Computer-Assisted
- Microscopy, Confocal
- Models, Neurological
- Neurons
(pathology)
- Stem Cell Transplantation
- Stem Cells
(physiology)
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