Abstract | AIM: METHODS: The cervical spondylosis model in rats was established by destroying the stability of cervical posterior column. The cord segments C4-6 and gastric antrum were collected 3 mo and 5 mo after the operation. Rats with the sham operation were used as controls. The expressions of c-Fos, caspase-3 and IL-1beta in the cervical cord and gastric antrum were determined by immunohistochemistry and/or Western blot. RESULTS: Immunohistochemical staining showed a few c-Fos, caspase-3 and IL-1beta-positive cells in the cervical cord and antrum in the control. There was a significant increase in c-Fos, caspase-3 and IL-1beta expression in model groups compared to the control groups at 3 mo and 5 mo after operation. More importantly, there was a significant (P < 0.05) increase in c-Fos, caspase-3 and IL-1beta expression in the model group rats at 3 mo compared to those at 5 mo after the operation (c-Fos: 11.20 +/- 2.26 vs 27.68 +/- 4.36 in the cervical cord, 11.3 +/- 2.3 vs 29.3 +/- 4.6 in the gastric antrum; caspase-3: 33.83 +/- 3.71 vs 36.32 +/- 4.01 in the cervical cord, 13.23 +/- 3.21 vs 26.32 +/- 4.01 in the gastric antrum; IL-1beta: 42.06 +/- 2.95 vs 45.91 +/- 3.98 in the cervical cord, 26.56 +/- 2.65 vs 32.01 +/- 2.98 in the gastric antrum). Western blot analysis showed time-dependent changes of caspase-3 and IL-1beta protein in the cervical cord and gastric antrum of rats with cervical spondylosis; there was no significant expression of caspase-3 and IL-1beta protein in the control group at 3 mo and 5 mo after the sham operation, whereas there was a significant difference in caspase-3 and IL-1beta protein levels between the model group rats followed up for 3 mo and those for 5 mo (P < 0.05). CONCLUSION: There is a significant association of c-Fos, caspase-3 and IL-1beta expressions in the gastric antrum with that in the spinal cord in rats with cervical spondylosis, suggesting that the gastrointestinal function may be affected by cervical spondylosis.
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Authors | Xing-Hua Song, Xiao-Xiong Xu, Li-Wen Ding, Li Cao, Alken Sadel, Hao Wen |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 13
Issue 18
Pg. 2575-80
(May 14 2007)
ISSN: 1007-9327 [Print] United States |
PMID | 17552004
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interleukin-1beta
- Proto-Oncogene Proteins c-fos
- Casp3 protein, rat
- Caspase 3
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Topics |
- Animals
- Caspase 3
(metabolism)
- Cervical Vertebrae
- Disease Models, Animal
- Dyspepsia
(etiology, pathology, physiopathology)
- Interleukin-1beta
(metabolism)
- Irritable Bowel Syndrome
(etiology, pathology, physiopathology)
- Pilot Projects
- Proto-Oncogene Proteins c-fos
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Spinal Osteophytosis
(complications, pathology, physiopathology)
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