Elastic plasma protein film blended with platelet releasate accelerates healing of diabetic mouse skin wounds.

Abstract

BACKGROUND AND OBJECTIVES:

The growth factors derived from platelets and plasma proteins mediate the wound-healing process that is characterized by the sequential migration and differentiation of several cell populations that give rise to angiogenesis, collagen synthesis, wound contraction, and re-epithelialization. To evaluate the efficacy of the blood-derived factors in wound healing, we examined a novel wound dressing consisting of concentrated human plasma proteins and platelet releasate (CPPP).

MATERIALS AND METHODS:

To generate CPPP, plasma proteins and platelets in the peripheral blood (n = 5) were concentrated with the cold ethanol precipitation method. The thrombin obtained from the same blood unit and calcium chloride (CaCl(2)) were mixed to a concentrate. The CPPP has enough strength to dress cutaneous wounds and contains large amounts of cytokines and fibronectin. We applied the CPPP to excisional skin wounds in genetically healing-impaired model mice (n= 5) and the wounds were evaluated 10 days after the operation.

RESULTS:

The area of CPPP-treated wounds decreased significantly compared with that of the control wounds (65% vs. 94% of the original size, respectively, P= 0.032). The immunostained section revealed a striking effect of CPPP on vascularization compared with the control wounds (13.2 vs. 2.7 vessels per mm(2) as mean vascular density observed in the sections, respectively, P= 0.013).

CONCLUSIONS:

Our results suggest that CPPP is a promising biologically active dressing for full-thickness skin wounds. CPPP can be an entirely autologous biological dressing, suggesting that it is free from the risk of transmission of pathogens through blood products.

• Authors: R Tanaka, S Ichioka, N Sekiya, N Ohura, S Uchino, A Ojima, Y Itoh, O Ishihara, T Nakatsuka, K Ikebuchi
• Journal: Vox sanguinis (Vox Sang) Vol. 93 Issue 1 Pg. 49-56 (Jul 2007) ISSN: 0042-9007 [Print] England
• PMID: 17547565 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Blood Proteins
• Cell Extracts
• Membranes, Artificial

Topics

• Animals
• Biological Dressings
• Blood Platelets (chemistry)
• Blood Proteins (therapeutic use)
• Cell Extracts (chemistry, therapeutic use)
• Diabetes Complications (pathology, therapy)
• Diabetes Mellitus, Type 2 (pathology, therapy)
• Disease Models, Animal
• Elasticity
• Humans
• Membranes, Artificial
• Mice
• Skin (injuries, pathology)
• Skin Diseases (pathology, therapy)
• Wound Healing
• Wounds and Injuries (pathology, therapy)