Abstract | PURPOSE: In a previous screen using a signal-trap library, we identified a number of secreted proteins up-regulated in primary tumor cells isolated from invasive breast cancers. The purpose of this study was to assess the expression of these genes in human invasive breast tumors and to determine the significance of their expression for prognosis in breast cancer. EXPERIMENTAL DESIGN: RESULTS: All five genes examined were expressed in invasive breast tumor cells. NTN4 protein expression was also confirmed by immunohistochemistry. Together, these data validate the design and screening of the signal-trap library. Univariate survival analysis revealed that expressions of TRA1, STC2, and NTN4 are correlated with longer disease-free survival and that TRA1 and NTN4 are associated with longer overall survival. Multivariate analysis showed that NTN4 is an independent prognostic factor of overall survival. CONCLUSIONS: This article describes the identification of three secreted proteins, NTN4, TRA1, and STC2, as potential novel prognostic markers in breast cancer.
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Authors | Selma Esseghir, Alan Kennedy, Pooja Seedhar, Ashutosh Nerurkar, Richard Poulsom, Jorge S Reis-Filho, Clare M Isacke |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 13
Issue 11
Pg. 3164-73
(Jun 01 2007)
ISSN: 1078-0432 [Print] United States |
PMID | 17545519
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- Biomarkers, Tumor
- Glycoproteins
- Intercellular Signaling Peptides and Proteins
- Membrane Glycoproteins
- NTN4 protein, human
- Nerve Growth Factors
- Netrins
- Protein Sorting Signals
- STC2 protein, human
- endoplasmin
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Topics |
- Antigens, Neoplasm
(metabolism)
- Biomarkers, Tumor
- Breast Neoplasms
(diagnosis, metabolism, pathology)
- Female
- Gene Expression Regulation, Neoplastic
- Glycoproteins
(biosynthesis)
- Humans
- Immunohistochemistry
- In Situ Hybridization
- Intercellular Signaling Peptides and Proteins
(biosynthesis)
- Membrane Glycoproteins
(biosynthesis)
- Nerve Growth Factors
(biosynthesis)
- Netrins
- Oligonucleotide Array Sequence Analysis
- Prognosis
- Protein Sorting Signals
- Treatment Outcome
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