[Comparative proteomic analysis of the prefrontal cortex between normal and heroin-addicted rats].

Abstract

OBJECTIVE:

To obtain two-dimensional gel electrophoresis maps of the prefrontal cortex (PFC) proteins of normal and heroin-addicted rats for identifying the differentially expressed proteins in the addicted rats.

METHODS:

Rat models of heroin addiction were established, and the proteins in the PFC underwent two-dimensional gel electrophoresis with immobiline pH gradient isoelectric focusing as the first and vertical SDS-PAGE as the second dimension. ImageMaster 2D 5.0 analysis software, matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS) or MALDI-TOF-TOF-MS as well as NCBInr database searching were performed to separate and identify the proteome in the rat PFC.

RESULTS:

Seven protein spots to represent 5 proteins were identified by PMF and MALDI-TOF-TOF-MS. Among those proteins, glucose-regulated protein (58 kD) and 26 S proteasome subunit p40.5 existed only in heroin-addicted rats, in which ATP synthase D chain was down-regulated. Ndufa10 and Eno1 were present in both groups, but their molecular mass and pI were different.

CONCLUSION:

Immobilized pH gradient two-dimensional gel electrophoresis allows good reproducibility in separation and identification of the proteome in the PDF of heroin-addicted rats, which facilitates further investigation of pathogenic mechanisms of heroin addiction and central nerve injury.

• Authors: Ping-ming Qiu, Hui-jun Wang, Chao Liu, Xue-feng Li, Xiao-hui Tan
• Journal: Nan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 27 Issue 4 Pg. 427-31 (Apr 2007) ISSN: 1673-4254 [Print] China
• PMID: 17545021 (Publication Type: English Abstract, Journal Article)

Chemical References

• Proteome
• Heroin

Topics

• Animals
• Electrophoresis, Gel, Two-Dimensional
• Electrophoresis, Polyacrylamide Gel
• Heroin (adverse effects)
• Image Processing, Computer-Assisted
• Prefrontal Cortex (drug effects, metabolism)
• Proteome (metabolism)
• Proteomics
• Rats
• Reproducibility of Results
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization