Immunohistochemical characterization of periodontal wound healing following nonsurgical treatment with fluorescence controlled Er:YAG laser radiation in dogs.

Abstract

BACKGROUND AND OBJECTIVE:

The aim of the present study was to immunohistochemically characterize periodontal wound healing following nonsurgical treatment with fluorescence controlled Er:YAG laser radiation in dogs.

STUDY DESIGN/MATERIALS AND METHODS:

Five beagle dogs suffering from naturally occurring chronic periodontitis were randomly allocated in a split-mouth design to nonsurgical periodontal treatment using either (a) an Er:YAG laser at 10.2, 12.8, 15.4, 18, and 20.4 J/cm2 (ERL1-5), or (b) an ultrasonic device (VUS) serving as control. The animals were sacrificed after 3 months. Histomorphometrical (e.g. inflammatory cell infiltrate, surface changes, cementum formation), and immunohistochemical parameters (collagen type I, CD68, matrix metalloproteinase (MMP)-8) were assessed.

RESULTS:

Inflammatory cell infiltrates of different extent were commonly observed in all treatment groups. However, histomorphometrical analysis revealed new cementum formation with inserting collagen type I fibers along the instrumented root surfaces in most specimens of both ERL (ERL2: 31+/-81 to ERL5: 595+/-575 microm) and VUS (50+/- 215 microm) groups. This was associated with pronounced CD68 and weak MMP-8 antigen reactivity.

CONCLUSION:

Within the limits of the present study, it was concluded that both treatment procedures (i) were effective in controlling inflammatory cell infiltrates, and (ii) may support the formation of a new connective tissue attachment.

• Authors: Frank Schwarz, Søren Jepsen, Monika Herten, Akira Aoki, Anton Sculean, Jürgen Becker
• Journal: Lasers in surgery and medicine (Lasers Surg Med) Vol. 39 Issue 5 Pg. 428-40 (Jun 2007) ISSN: 0196-8092 [Print] United States
• PMID: 17523168 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: (c) 2007 Wiley-Liss, Inc.

Chemical References

• Antigens, CD
• Antigens, Differentiation, Myelomonocytic
• CD68 antigen, human
• Collagen Type I
• Matrix Metalloproteinase 8

Topics

• Animals
• Antigens, CD (analysis)
• Antigens, Differentiation, Myelomonocytic (analysis)
• Cementogenesis
• Collagen Type I (metabolism)
• Dogs
• Female
• Fluorescence
• Immunohistochemistry
• Lasers
• Macrophages (pathology)
• Matrix Metalloproteinase 8 (analysis)
• Periodontitis (therapy)
• Plasma Cells (pathology)
• Random Allocation
• Staining and Labeling
• Wound Healing