Abstract | BACKGROUND: OBJECTIVE: Our aim was to better understand and to advance these encouraging transplantation results. DESIGN: In 1999, based on emerging mouse transplantation models involving implanted donor bone fragments as well as osteoblast-like cells cultured from bone, we treated a 9-month-old girl suffering a similar course of infantile HPP. RESULTS: Four months later, radiographs demonstrated improved skeletal mineralization. Twenty months later, PCR analysis of adherent cells cultured from recipient bone suggested the presence of small amounts of paternal (donor) DNA despite the absence of hematopoietic engraftment. This patient, now 8 yr old (7 yr after transplantation), is active and growing, and has the clinical phenotype of the more mild, childhood form of HPP. CONCLUSIONS: Cumulative experience suggests that, after immune tolerance, donor bone fragments and marrow may provide precursor cells for distribution and engraftment in the skeletal microenvironment in HPP patients to form tissue-nonspecific isoenzyme of alkaline phosphatase-replete osteoblasts that can improve mineralization.
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Authors | Richard A Cahill, Deborah Wenkert, Sharon A Perlman, Ann Steele, Stephen P Coburn, William H McAlister, Steven Mumm, Michael P Whyte |
Journal | The Journal of clinical endocrinology and metabolism
(J Clin Endocrinol Metab)
Vol. 92
Issue 8
Pg. 2923-30
(Aug 2007)
ISSN: 0021-972X [Print] United States |
PMID | 17519318
(Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Alkaline Phosphatase
(blood, genetics)
- Bone Marrow Transplantation
- Bone Transplantation
- Cell Differentiation
(physiology)
- Cell Proliferation
- Cells, Cultured
- Child
- Cytogenetics
- DNA
(genetics)
- Female
- Humans
- Hypercalcemia
(etiology)
- Hypophosphatasia
(complications, therapy)
- Infant
- Knee
(diagnostic imaging)
- Osteoblasts
(transplantation)
- Radiography
- Reverse Transcriptase Polymerase Chain Reaction
- Stem Cells
(physiology)
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