Maternal allergy influences the proliferation of neonatal T cells expressing CCR4, CXCR5 or CD103.

Abstract

BACKGROUND:

Elevated proliferative response to allergen in cord blood mononuclear cells (CBMCs) is related to subsequent allergy development of the neonate and has been suggested as a screening marker for high allergy risk.

OBJECTIVE:

To characterize the proliferating cells in CBMCs from a neonatal group influenced by maternal allergy compared with a control group without known allergic heredity.

METHODS:

CBMCs were stimulated with bovine beta-lactoglobulin (beta-LG) and proliferation was analysed by radioactive thymidine incorporation and expressed both as the traditional stimulation index (SI) and SI corrected by eliminating non-specific proliferation. After beta-LG combined with endotoxin stimulation, cellular expression of IL-4 and IFN-gamma mRNA was determined by quantitative RT-PCR and adhesion as well as chemokine receptors were analysed by three-colour flow cytometry in proliferating T cells (CD3+ Ki-67+).

RESULTS:

The percentage of CCR4+ cells correlated weakly with concurrent IL-4 expression (r(S)=0.5, P<0.05), while CXCR3 correlated strongly with IFN-gamma expression (r(S)=0.83, P<0.001). In the allergy risk group, the percentage of proliferating T cells expressing CCR4 or integrin alphaE (CD103) was significantly reduced compared with the control group, while CXCR5 and the corrected SI were relatively increased (CCR4: P=0.01; integrin alphaE: P=0.03; CXCR5: P=0.04; SI: P=0.04).

CONCLUSION:

Our results implied delayed maturation of immune functions involved in cellular migration, cell-cell interaction and immunoregulatory functions in neonates with hereditary allergy risk. The alterations observed in this subject group suggested that the corrected SI as well as proliferation of CCR4+, CXCR5+ or CD103+ T cells in allergen-stimulated CBMCs might serve as early screening markers for allergy risk.

• Authors: U Haddeland, P Brandtzaeg, B Nakstad
• Journal: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology (Clin Exp Allergy) Vol. 37 Issue 6 Pg. 856-64 (Jun 2007) ISSN: 0954-7894 [Print] England
• PMID: 17517099 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Antigens, CD
• CCR4 protein, human
• CD3 Complex
• CXCR5 protein, human
• Endotoxins
• IL4 protein, human
• Integrin alpha Chains
• Ki-67 Antigen
• Lactoglobulins
• Receptors, CCR4
• Receptors, CXCR5
• Receptors, Chemokine
• alpha E integrins
• Interleukin-4
• Interferon-gamma

Topics

• Adult
• Allergens (immunology, pharmacology)
• Animals
• Antigens, CD (biosynthesis, immunology)
• CD3 Complex (immunology)
• Cattle
• Cell Communication (drug effects, immunology)
• Cell Movement (drug effects, immunology)
• Cell Proliferation (drug effects)
• Endotoxins (pharmacology)
• Female
• Fetal Blood (immunology, metabolism)
• Flow Cytometry
• Gene Expression Regulation (drug effects, immunology)
• Humans
• Hypersensitivity (congenital, etiology, immunology, metabolism)
• Infant, Newborn
• Integrin alpha Chains (biosynthesis, immunology)
• Interferon-gamma (immunology)
• Interleukin-4 (immunology)
• Ki-67 Antigen (immunology)
• Lactoglobulins (immunology, pharmacology)
• Male
• Maternal-Fetal Exchange (immunology)
• Pregnancy
• Receptors, CCR4
• Receptors, CXCR5
• Receptors, Chemokine (biosynthesis, immunology)
• T-Lymphocytes (immunology, metabolism)