A cytosolic NADP-malic enzyme gene from rice (Oryza sativa L.) confers salt tolerance in transgenic Arabidopsis.

Abstract

NADP-malic enzyme (NADP-ME, EC 1.1.1.40) functions in many different pathways in plant and may be involved in plant defense such as wound and UV-B radiation. Here, expression of the gene encoding cytosolic NADP-ME (cytoNADP-ME, GenBank Accession No. AY444338) in rice (Oryza sativa L.) seedlings was induced by salt stress (NaCl). NADP-ME activities in leaves and roots of rice also increased in response to NaCl. Transgenic Arabidopsis plants over-expressing rice cytoNADP-ME had a greater salt tolerance at the seedling stage than wild-type plants in MS medium-supplemented with different levels of NaCl. Cytosolic NADPH/NADP(+) concentration ratio of transgenic plants was higher than those of wild-type plants. These results suggest that rice cytoNADP-ME confers salt tolerance in transgenic Arabidopsis seedlings.

• Authors: Yuxiang Cheng, Mei Long
• Journal: Biotechnology letters (Biotechnol Lett) Vol. 29 Issue 7 Pg. 1129-34 (Jul 2007) ISSN: 0141-5492 [Print] Netherlands
• PMID: 17516134 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Plant
• Sodium Chloride
• NADP
• Malate Dehydrogenase
• malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+)

Topics

• Adaptation, Physiological (drug effects)
• Arabidopsis (drug effects, genetics, metabolism)
• Cytosol (drug effects, enzymology)
• DNA, Plant (metabolism)
• Gene Expression Regulation, Plant (drug effects)
• Genome, Plant (genetics)
• Malate Dehydrogenase (genetics, metabolism)
• NADP (metabolism)
• Oryza (enzymology, genetics)
• Plant Leaves (drug effects, enzymology)
• Plant Roots (drug effects, enzymology)
• Plants, Genetically Modified
• Seedlings (drug effects, metabolism)
• Sodium Chloride (pharmacology)
• Time Factors
• Transformation, Genetic (drug effects)