Inhibition by tyroserleutide (YSL) on the invasion and adhesion of the mouse melanoma cell.

Abstract

Tyroserleutide (YSL) is an active, low-molecular-weight polypeptide, comprised of three amino acids, that has shown antitumor effects on human hepatocarcinoma BEL-7402 in vitro and in vivo. In this study, we evaluated the inhibition of YSL on invasion and adhesion of the mouse B16-F10 melanoma cell line by injecting B16-F10 cells into the tail veins of C57BL/6 mice to establish an experimental lung metastasis model. YSL inhibited B16-F10 cell metastasis to lung, reducing the number and area of metastasis lesions. When we treated B16-F10 cells with YSL (0.01, 0.1, 1, 10, or 100 microg/mL) in vitro, we found that YSL inhibited the proliferation of B16-F10 cells with a 28.11% rate of inhibition. YSL significantly decreased the adhesiveness of B16-F10 cells to Matrigel with a 29.15% inhibition rate; YSL also significantly inhibited the invasion of B16-F10 cells, producing an inhibition of 35.31%. By analyses with Western blot and real-time RT-PCR, we found that YSL markedly inhibited the expression of ICAM-1 in B16-F10 cells. These data suggest that YSL inhibits the growth, invasion, and adhesion of B16-F10 cells.

• Authors: Zhi Yao, Xu-Chun Che, Rong Lu, Min-Na Zheng, Zhi-Feng Zhu, Jin-Ping Li, Xu Jian, Lin-Xi Shi, Jun-Yan Liu, Wen-Yuan Gao
• Journal: Molecular medicine (Cambridge, Mass.) (Mol Med) 2007 Jan-Feb Vol. 13 Issue 1-2 Pg. 14-21 ISSN: 1076-1551 [Print] England
• PMID: 17515953 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Drug Combinations
• Laminin
• Oligopeptides
• Proteoglycans
• matrigel
• Collagen
• H-Tyr-Ser-Leu-OH

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Blotting, Western
• Cell Adhesion (drug effects)
• Cell Proliferation (drug effects)
• Collagen (metabolism)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Drug Combinations
• Drug Evaluation, Preclinical
• Female
• Laminin (metabolism)
• Lung Neoplasms (pathology, secondary)
• Melanoma, Experimental (pathology)
• Mice
• Mice, Inbred C57BL
• Molecular Weight
• Neoplasm Invasiveness (prevention & control)
• Neoplasm Transplantation
• Oligopeptides (chemistry, pharmacology)
• Proteoglycans (metabolism)
• Random Allocation
• Reverse Transcriptase Polymerase Chain Reaction
• Transplantation, Homologous
• Tumor Cells, Cultured