Measles virus-specific plasma cells are prominent in subacute sclerosing panencephalitis CSF.

Abstract

OBJECTIVE:

To demonstrate the specificity of expanded CD138(+) plasma cell clones recovered from the CSF of a patient with subacute sclerosing panencephalitis (SSPE) for measles virus (MV).

METHODS:

IgG variable region sequences of single-antibody-secreting CD138(+) cells sorted from SSPE CSF were amplified by single-cell PCR and analyzed. Human IgG1 recombinant antibodies (rAbs) were produced from four expanded CD138(+) clones and assayed for immunoreactivity against MV proteins.

RESULTS:

Clonal expansion was a prominent feature of the SSPE plasma cell repertoire, and each of the four rAbs assayed was specific for either the MV fusion or the MV nucleocapsid protein.

CONCLUSIONS:

Expanded plasma cell clones in the CSF of patients with subacute sclerosing panencephalitis produce disease-relevant antibodies. Recombinant antibodies derived from CSF B cells could provide a tool to identify target antigens in idiopathic inflammatory disorders.

• Authors: G P Owens, A M Ritchie, D H Gilden, M P Burgoon, D Becker, J L Bennett
• Journal: Neurology (Neurology) Vol. 68 Issue 21 Pg. 1815-9 (May 22 2007) ISSN: 1526-632X [Electronic] United States
• PMID: 17515543 (Publication Type: Case Reports, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Immunoglobulin G
• Nucleocapsid Proteins
• Recombinant Fusion Proteins
• Viral Fusion Proteins
• Viral Proteins

Topics

• Antibodies, Viral (cerebrospinal fluid, immunology)
• Antibody Specificity (immunology)
• Cell Separation
• Cells, Cultured
• Child
• Clone Cells (immunology)
• Humans
• Immunoglobulin G (cerebrospinal fluid, genetics, immunology)
• Male
• Measles virus (immunology)
• Nucleocapsid Proteins (immunology)
• Plasma Cells (immunology, virology)
• Recombinant Fusion Proteins (cerebrospinal fluid, immunology)
• Subacute Sclerosing Panencephalitis (cerebrospinal fluid, immunology, virology)
• Viral Fusion Proteins (immunology)
• Viral Proteins (immunology)