Cytotoxic and apoptosis-inducing properties of auriculoside A in tumor cells.

Abstract

The C21-steroidal glycoside auriculoside A (1), recently isolated from the roots of Cynanchum auriculatum, was found to inhibit the growth of several human tumor cell lines and to induce apoptosis in human breast cancer (MCF-7) cells. Compound 1 was evaluated for its in vitro cytotoxicity against MCF-7, HO-8910, and Bel-7402 cells, and for its in vivo antitumor effects on implanted sarcoma-180 (S180) tumors in mice. It showed significant, concentration-dependent inhibition of the cancer cells, both in vitro and in vivo. MCF-7 Cells exposed to 1 displayed typical morphological apoptosis characteristics such as cytoplasm contraction and nuclear-chromatin condensation. Flow-cytometric analysis showed that the MCF-7 cell cycle was arrested at the G0/G1 phase. When treated with 40 microg/ml of 1 for 24, 48, and 72 h, respectively, the apoptotic rates of the cells were ca. 5, 8, and 18.5%, respectively.

• Authors: Rusong Zhang, Yulan Liu, Yiqi Wang, Yiping Ye, Xiaoyu Li
• Journal: Chemistry & biodiversity (Chem Biodivers) Vol. 4 Issue 5 Pg. 887-92 (May 2007) ISSN: 1612-1880 [Electronic] Switzerland
• PMID: 17511004 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 3-O-(glucopyranosyl-1-4-cymaropyranosyl-1-4-cymaropyranosyl-1-4-oleandropyranosyl-1-4-cymaropyranosyl)caudatin
• Antineoplastic Agents
• Saponins

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects, physiology)
• Cell Line, Tumor
• Dose-Response Relationship, Drug
• Humans
• Mice
• Saponins (pharmacology)
• Sarcoma 180