Hepatitis C virus induces proteolytic cleavage of sterol regulatory element binding proteins and stimulates their phosphorylation via oxidative stress.

Abstract

Hepatic steatosis is a common histological feature of chronic hepatitis C. Hepatitis C virus (HCV) gene expression has been shown to alter host cell cholesterol/lipid metabolism and thus induce hepatic steatosis. Since sterol regulatory element binding proteins (SREBPs) are major regulators of lipid metabolism, we sought to determine whether genotype 2a-based HCV infection induces the expression and posttranslational activation of SREBPs. HCV infection stimulates the expression of genes related to lipogenesis. HCV induces the proteolytic cleavage of SREBPs. HCV core and NS4b derived from genotype 3a are also individually capable of inducing the proteolytic processing of SREBPs. Further, we demonstrate that HCV stimulates the phosphorylation of SREBPs. Our studies show that HCV-induced oxidative stress and subsequent activation of the phosphatidylinositol 3-kinase (PI3-K)-Akt pathway and inactivation (phosphorylation) of PTEN (phosphatase and tensin homologue) mediate the transactivation of SREBPs. HCV-induced SREBP-1 and -2 activities were sensitive to antioxidant (pyrrolidine dithiocarbamate), Ca(2+) chelator 1,2-bis(aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-tetra(acetoxymethyl) ester (BAPTA-AM), and PI3-K inhibitor (LY294002). Collectively, these studies provide insight into the mechanisms of hepatic steatosis associated with HCV infection.

• Authors: Gulam Waris, Daniel Jeffery Felmlee, Francesco Negro, Aleem Siddiqui
• Journal: Journal of virology (J Virol) Vol. 81 Issue 15 Pg. 8122-30 (Aug 2007) ISSN: 0022-538X [Print] United States
• PMID: 17507484 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Retracted Publication)

Chemical References

• Protein Isoforms
• Sterol Regulatory Element Binding Proteins
• Phosphatidylinositol 3-Kinases
• PTEN Phosphohydrolase

Topics

• Animals
• Cell Line
• Enzyme Activation
• Fatty Liver (pathology, virology)
• Gene Expression Regulation
• Genes, Reporter
• Hepacivirus (metabolism)
• Hepatitis C, Chronic (metabolism)
• Humans
• Lipid Metabolism
• Oxidative Stress
• PTEN Phosphohydrolase (metabolism)
• Phosphatidylinositol 3-Kinases (metabolism)
• Phosphorylation
• Protein Isoforms (genetics, metabolism)
• Signal Transduction (physiology)
• Sterol Regulatory Element Binding Proteins (genetics, metabolism)