HIV-1 prophylactic vaccine comprised of topical DermaVir prime and protein boost elicits cellular immune responses and controls pathogenic R5 SHIV162P3.
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| Abstract |
Topical DNA vaccination (DermaVir) facilitates antigen presentation to naive T cells. DermaVir immunization in mice, using HIV-1 Env and Gag, elicited cellular immune responses. Boosting with HIV-1 gp120 Env and p41 Gag augmented Th1 cytokine levels. Intramuscular DNA administration was less efficient in priming antigen-specific cytokine production and memory T cells. In rhesus macaques, DermaVir immunization induced Gag- and Env-specific Th1 and Th2 cytokines and generation of memory T cells. Boosting of DermaVir-primed serum antibody levels was noted following gp140(SHIV89.6P)/p27(SIV) immunization. Rectal challenge with pathogenic R5-tropic SHIV162P3 resulted in control of plasma viremia (4/5 animals) that was reflected in jejunum, colon and mesenteric lymph nodes. An inverse correlation was found between Gag- and Env-specific central memory T cell responses on the day of challenge and plasma viremia at set point. Overall, the topical DermaVir/protein vaccination yields central memory T cell responses and facilitates control of pathogenic SHIV infection.
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| Authors | Anthony D Cristillo, Julianna Lisziewicz, Leilei He, Franco Lori, Lindsey Galmin, Jeffrey N Trocio, Tami Unangst, Lucia Whitman, Lauren Hudacik, Nyasha Bakare, Stephen Whitney, Susana Restrepo, John Suschak, Maria Grazi Ferrari, H K Chung, Vaniambadi S Kalyanaraman, Phillip Markham, Ranajit Pal |
| Journal | Virology (Virology) Vol. 366 Issue 1 Pg. 197-211 (Sep 15 2007) ISSN: 0042-6822 [Print] United States |
| PMID | 17499328 (Publication Type: Journal Article) |
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