Lipopolysaccharide (LPS) induces
acute lung injury (ALI) via
Toll-like receptor 4 (TLR4)-mediated MAPK activation. The
lipid A fraction of LPS is considered to be the active moiety, but whether the
lipid A-TLR4 interaction accounts completely for ALI-associated MAPK activation in vivo has not been determined. The
lipid A fraction of LPS induces a discrete MAPK activation pattern in murine ALI. Mice (C57BL/6J, C3H/HeJ) were treated with intratracheal instillations of purified
lipid A or LPS (10, 30, and 100 microg per mouse) or vehicle. ALI was assessed by histology. Chromogenic
myeloperoxidase (MPO) activity was measured in lung homogenates. MAPK expression was quantified by immunoblotting. In vitro ERK inhibitor studies using thioglycollate-elicited macrophages were also performed. MPO increased in a dose- and time-responsive fashion. Notably, MPO was 2.4-fold greater after
lipid A compared with LPS and vehicle at 6 h after instillation (
lipid A, 0.88 +/- 0.25 vs. LPS, 0.37 +/- 0.21 optical density units.min(-1).mg(-1); P < 0.05). However, ALI scores were comparable at 6 and 24 h between LPS and
lipid A. MPO was also comparable in vehicle-treated or C3H/HeJ mice treated with LPS or
lipid A at 6 and 24 h. Phospho-ERK activation was pronounced at 6 and 24 h after
lipid A but not LPS treatment. In vitro studies confirmed the relationship between phospho-ERK activation and
cytokine expression in macrophage stimulated with either LPS or
lipid A. Compared with whole LPS, the
lipid A fraction is associated with amplified whole lung MPO and ERK activation 6 h after intratracheal instillation in mice.