An improved method for the determination of acetylcholinesterase activity in rectal biopsy tissue from patients with Hirschsprung's disease.

Abstract

A method is described for the assay of acetylcholinesterase in tissue samples using the selective cholinesterase inhibitor, lysivane (10-(alpha-diethylaminopropyl) phenothiazine hydrochloride). Significantly increased enzyme activity is found in rectal biopsy specimens from patients with Hirschsprung's disease (p less than 0.001) indicating the diagnostic usefulness of the assay.

• Authors: G Dale, J R Bonham, K W Riley, J Wagget
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 77 Issue 3 Pg. 407-13 (Jun 15 1977) ISSN: 0009-8981 [Print] Netherlands
• PMID: 17496 (Publication Type: Journal Article)

Chemical References

• Cholinesterase Inhibitors
• Phenothiazines
• Acetylcholinesterase
• Cholinesterases

Topics

• Acetylcholinesterase (metabolism)
• Adolescent
• Child
• Child, Preschool
• Cholinesterase Inhibitors
• Cholinesterases (metabolism)
• Female
• Humans
• Infant
• Infant, Newborn
• Male
• Megacolon (enzymology)
• Methods
• Phenothiazines
• Rectum (enzymology)