Fractional incorporation of radionucleotides, a marker of in vitro tumor cell chemosensitivity in colorectal cancer.

Abstract

Working with the antimetabolic chemopredictivity assay on short term cultures we evidenced a correlation between in vitro chemosensitivity of colorectal cancer and fractional incorporation of radionucleotides. Four different drugs (5-FU, mitomycin C, 4'-iododeoxydoxorubicin and ruboxyl, a nitroxyl derivative of daunorubicin) were tested on 102 tumor cultures. The extent of 3H-TdR and 3H-UdR incorporation into DNA and RNA in the related control cultures was in relationship with the chemosensitivity of the tumor. Along with the labeling index this simple metabolic-kinetic trait may gain a role for the screening of tumor phenotypes sensitive to chemotherapy.

• Authors: F Franchi, P Seminara, D Giannarelli, N Konovalova
• Journal: Oncology (Oncology) Vol. 48 Issue 6 Pg. 510-6 ( 1991) ISSN: 0030-2414 [Print] Switzerland
• PMID: 1749592 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• DNA, Neoplasm
• RNA, Neoplasm
• Tritium
• Mitomycin
• 4'-deoxy-4'-iododoxorubicin
• Doxorubicin
• ruboxyl
• Thymidine
• Uridine
• Daunorubicin

Topics

• Antineoplastic Agents (pharmacology)
• Colorectal Neoplasms (drug therapy, metabolism)
• DNA, Neoplasm (biosynthesis)
• Daunorubicin (analogs & derivatives, pharmacology)
• Doxorubicin (analogs & derivatives, pharmacology)
• Humans
• In Vitro Techniques
• Liver Neoplasms (secondary)
• Mitomycin (pharmacology)
• RNA, Neoplasm (biosynthesis)
• Thymidine (metabolism)
• Tritium
• Tumor Cells, Cultured (drug effects, metabolism)
• Uridine (metabolism)