Determination of thymosin beta4 and protein in human wound fluid after abdominal surgery.

Abstract

Wound fluids were collected up to 60 h after abdominal surgery. Immediately after obtaining the wound fluid by Robinson drainage, wound fluid was centrifuged to remove blood cells and inflammatory cells. The concentration of total protein as well as of thymosin beta(4) was determined in the cell-free supernatant solution. Total protein concentration decreased from about 50 g/L to 30 g/L within 60 h after surgery. After surgery we observed a concentration of up to 20 mg thymosin beta(4) per liter decreasing to about 1 mg/L with time. Neither thymosin beta(10) nor oxidized thymosin beta(4) was detected in human wound fluid.

• Authors: S Bodendorf, G Born, E Hannappel
• Journal: Annals of the New York Academy of Sciences (Ann N Y Acad Sci) Vol. 1112 Pg. 418-24 (Sep 2007) ISSN: 0077-8923 [Print] United States
• PMID: 17495244 (Publication Type: Journal Article)

Chemical References

• Proteins
• thymosin beta(4)
• Thymosin

Topics

• Abdomen (surgery)
• Chromatography, High Pressure Liquid
• Humans
• Postoperative Complications (pathology)
• Proteins (analysis)
• Surgical Procedures, Operative (adverse effects)
• Thymosin (analysis)
• Wounds and Injuries (pathology)