Measurements of desmosine and isodesmosine by mass spectrometry in COPD.

Abstract

OBJECTIVES:

Application of mass spectrometry (MS) for direct measurements of desmosine (D) and isodesmosine (I) in urine, plasma, and sputum as markers of elastin degradation in patients with alpha(1)-antitrypsin deficiency (AATD) and non-AATD-related COPD.

BACKGROUND:

In COPD patients, the lungs undergo elastin injury, which can be monitored by measurements of D and I in body fluids as specific markers of elastin degradation using the specificity and sensitivity of MS.

METHODS:

Acid hydrolysis of blood plasma, 24-h urine and sputum measurements, followed by chromatographic separation for mass spectrometric analysis.

RESULTS:

Each patient group had levels of plasma D and I that were statistically significantly higher than those of control subjects. AATD patients had higher levels than COPD patients with normal alpha(1)-antitrypsin (AAT) levels. Twenty-four-hour urine measurements demonstrated no significant difference in total levels of D and I among control subjects and patients but showed a free (unbound) concentration of D and I in urine, which was statistically significantly higher in patients with COPD with and without AAT. The D and I levels in the sputum of patients with AATD exceeded the levels in COPD patients with normal AAT levels.

CONCLUSIONS:

MS allows a sensitive and specific analysis of D and I in body fluids. The quantification of D and I in sputum, along with increases of D and I in plasma and an elevated free component of D and I in urine provide indexes that characterize patients with COPD and can be followed in relation to the course of the disease and/or therapy.

• Authors: Shuren Ma, Yong Y Lin, Gerard M Turino
• Journal: Chest (Chest) Vol. 131 Issue 5 Pg. 1363-71 (May 2007) ISSN: 0012-3692 [Print] United States
• PMID: 17494786 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Desmosine
• Elastin
• Isodesmosine

Topics

• Adult
• Aged
• Aged, 80 and over
• Desmosine (analysis)
• Elastin (analysis)
• Female
• Humans
• Isodesmosine (analysis)
• Male
• Mass Spectrometry
• Matched-Pair Analysis
• Middle Aged
• Prognosis
• Pulmonary Disease, Chronic Obstructive (blood, classification, urine)
• Sensitivity and Specificity
• Sputum (chemistry)
• alpha 1-Antitrypsin Deficiency (metabolism)