Abstract |
The post-transcriptional control of mRNA levels is a very powerful mechanism which allows cells to quickly change the amount of specific proteins. In this study, we wanted to analyze whether the Brn-3b transcription factor, essential for the proper development of mouse retinal ganglion cells, is subjected to such post-transcriptional regulation. In particular, due to its conservation amongst different species, we wanted to study the role of its 3' untranslated region ( 3'UTR). We show that the 3'UTR of the Brn-3b mRNA does indeed contain regulatory sequences that mediate mRNA degradation upon serum starvation-induced differentiation of ND7 neuroblastoma cells. The specific region mediating this effect has been characterized and two different microRNAs that potentially regulate the stability of Brn-3b have been identified. Moreover we show that Dicer, one of the key enzymes in the production of microRNAs, is strongly up-regulated in ND7 cells subjected to differentiation.
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Authors | Mattia Calissano, James K J Diss, David S Latchman |
Journal | FEBS letters
(FEBS Lett)
Vol. 581
Issue 13
Pg. 2490-6
(May 29 2007)
ISSN: 0014-5793 [Print] England |
PMID | 17490655
(Publication Type: Journal Article)
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Chemical References |
- 3' Untranslated Regions
- MicroRNAs
- RNA, Messenger
- RNA, Neoplasm
- Transcription Factor Brn-3A
- Transcription Factor Brn-3B
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Topics |
- 3' Untranslated Regions
(genetics)
- Blotting, Northern
- Breast Neoplasms
- Cell Line, Tumor
- Female
- Gene Expression Regulation, Neoplastic
- Genetic Vectors
- Humans
- MicroRNAs
(genetics)
- Neuroblastoma
- RNA Processing, Post-Transcriptional
- RNA, Messenger
(genetics)
- RNA, Neoplasm
(genetics, isolation & purification)
- Reverse Transcriptase Polymerase Chain Reaction
- Transcription Factor Brn-3A
(genetics)
- Transcription Factor Brn-3B
(genetics)
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