High rabdosiin and rosmarinic acid production in Eritrichium sericeum callus cultures and the effect of the calli on masugi-nephritis in rats.

Abstract

During an investigation of plant cell cultures that might be useful in the treatment of renal disorders, we established a vigorously-growing E-4 callus culture of Eritrichium sericeum that produced large amounts of caffeic acid metabolites, (-)-rabdosiin (1.8% dry wt) and rosmarinic acid (4.6% dry wt). Elicitation of the calli by methyl jasmonate induced a 38% increase in total polyphenol production. The most efficient method of eliciting (-)-rabdosiin biosynthesis was through the treatment of E-4 calli with cuprum glycerate, which induced an increase in (-)-rabdosiin production of as much as 4.1% dry wt. Oral administration of E-4 callus biomass (100 mg/kg/d for 30 d) to rats with induced Masugi-nephritis caused an increase in diuresis and lowered creatinine excretion and proteinuria levels as compared with Masugi-nephritis untreated rats. While all of the Masugi-nephritis untreated rats began to suffer, near a quarter of the E-4 treated rats remained in good health. This result indicates that the E-4 culture has the potential to alleviate the symptoms associated with nephritis.

• Authors: Yuliya V Inyushkina, Victor P Bulgakov, Marina V Veselova, Valery M Bryukhanov, Yakov F Zverev, Vyacheslav V Lampatov, Olga V Azarova, Galina K Tchernoded, Sergey A Fedoreyev, Yury N Zhuravlev
• Journal: Bioscience, biotechnology, and biochemistry (Biosci Biotechnol Biochem) Vol. 71 Issue 5 Pg. 1286-93 (May 2007) ISSN: 0916-8451 [Print] England
• PMID: 17485828 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Acetates
• Caffeic Acids
• Cinnamates
• Cyclopentanes
• Depsides
• Glyceric Acids
• Lignans
• Oxylipins
• Plant Growth Regulators
• rabdosiin
• glyceric acid
• Copper
• methyl jasmonate
• Creatinine
• rosmarinic acid

Topics

• Acetates (pharmacology)
• Animals
• Biomass
• Boraginaceae (chemistry, cytology, drug effects, metabolism)
• Caffeic Acids (chemistry, metabolism)
• Cells, Cultured
• Cinnamates (chemistry, metabolism)
• Copper (pharmacology)
• Creatinine (metabolism)
• Cyclopentanes (pharmacology)
• Depsides (chemistry, metabolism)
• Diuresis (drug effects)
• Glyceric Acids (pharmacology)
• Kinetics
• Lignans
• Molecular Structure
• Nephritis (chemically induced, classification, drug therapy, pathology, physiopathology)
• Oxylipins
• Phytotherapy
• Plant Growth Regulators (pharmacology)
• Proteinuria (drug therapy)
• Random Allocation
• Rats
• Rats, Sprague-Dawley