Abstract | BACKGROUND: METHODS: Experimental periodontitis was induced in New Zealand white rabbits with silk sutures tied around the mandibular second premolars bilaterally, followed by the topical application of 10(9) colony forming units (CFU) of P. gingivalis. 1-Tetradecanol complex (1-TDC) was topically applied at 1- and 10-mg/ml concentrations in five animals in each group, whereas control animals received olive oil vehicle (five animals) three times per week for 6 weeks. Negative controls included ligature alone (14 animals) or ligature + P. gingivalis (non-treatment; 15 animals). Rabbits were sacrificed after 6 weeks, and mandibular block sections were obtained; tissues were decalcified and embedded in paraffin. Thin sections (5 microm) were stained with hematoxylin and eosin or tartrate-resistant acid phosphatase. Macroscopic and histologic evaluation of samples was followed by the characterization of cellular inflammatory infiltrate and quantitative histomorphometric measurements. RESULTS: Treatment with both concentrations of 1-TDC and vehicle resulted in significant prevention of macroscopic periodontal inflammation and bone loss (75%; P <0.05) compared to the non-treatment ( ligature + P. gingivalis) group, where significant periodontal tissue destruction characterized by attachment and bone loss was detected. However, there was no statistically significant difference between the vehicle and both 1-TDC groups. Histologically, 1-TDC inhibited inflammatory cell infiltration and prevented osteoclastogenesis, whereas treatment with vehicle did not show the same effect as in the 1-TDC groups; the difference between vehicle and the higher concentration of 1-TDC (10 mg/ml) was statistically significant. CONCLUSION: Topical application of an esterified monounsaturated fatty acid complex (1-TDC) was found promising in preventing bone loss, inflammatory cell infiltration, and connective tissue destruction in the rabbit periodontitis model.
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Authors | Hatice Hasturk, Victor L Jones, Chris Andry, Alpdogan Kantarci |
Journal | Journal of periodontology
(J Periodontol)
Vol. 78
Issue 5
Pg. 924-32
(May 2007)
ISSN: 0022-3492 [Print] United States |
PMID | 17470028
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Fatty Acids, Monounsaturated
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Topics |
- Administration, Topical
- Alveolar Bone Loss
(metabolism, microbiology, prevention & control)
- Animals
- Dose-Response Relationship, Drug
- Esterification
- Fatty Acids, Monounsaturated
(administration & dosage, pharmacology)
- Male
- Mandible
- Periodontal Attachment Loss
(metabolism, microbiology, prevention & control)
- Periodontitis
(metabolism, microbiology, prevention & control)
- Porphyromonas gingivalis
(drug effects, pathogenicity)
- Rabbits
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