Abstract |
Recent studies suggested that glycosylphosphatidylinositol-specific phospholipase D ( GPI-PLD) correlated with tumor malignancy and prognosis of certain tumors. As hematopoietic stem/progenitor cells (HS/PC) homing was similar to tumor invasion and metastasis in some mechanisms, which arose our interests in whether GPI-PLD contribution to the homing of HS/PC. In this study, CD34(+) cells from umbilical cord blood (UCB), mobilized peripheral blood (MPB), and bone marrow (BM) were assayed for their differences in adhesion, migration, respectively. The expression of GPI-anchored proteins (CD48, CD90) on the cells were analyzed by flow cytometry. Semi-quantitive RT-PCR was used to detect GPI-PLD expression in the three different CD34(+) cells. The results showed that GPI-PLD had no effect on the adhesion of CD34(+) cells. While, spontaneous and SDF-1 induced migration of UCB and MPB, but not BM CD34(+) cells were decreased after 1,10-phenanthroline (an inhibitor of GPI-PLD) pretreatment. Furthermore, we found little difference in GPI-anchored adhesion molecules (CD48, CD90) expression between untreated and pretreated CD34(+) cells. GPI-PLD mRNA was low expressed in MPB and undetected in UCB and BM CD34(+) cells. Our results suggested that GPI-PLD probably had no contribution to HS/PC homing, which may due to its low or no expression in UCB, BM and MPB CD34(+) cells.
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Authors | Kui Song, Xiaojuan Sun, Jun Wang, Shujuan Zhou, Hui Zeng, Fangping Chen |
Journal | Leukemia research
(Leuk Res)
Vol. 31
Issue 12
Pg. 1701-7
(Dec 2007)
ISSN: 0145-2126 [Print] England |
PMID | 17467053
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, CD
- Antigens, CD34
- CD48 Antigen
- CD48 protein, human
- Thy-1 Antigens
- Phospholipase D
- glycoprotein phospholipase D
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Topics |
- Antigens, CD
(analysis)
- Antigens, CD34
- Bone Marrow Cells
- CD48 Antigen
- Cell Movement
- Fetal Blood
- Hematopoietic Stem Cells
(physiology)
- Humans
- Phospholipase D
(analysis, physiology)
- Reverse Transcriptase Polymerase Chain Reaction
- Thy-1 Antigens
(analysis)
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