[Influence of the VEGF antibody targeted vascular therapy on the expression of collagen type I in hyperplastic].

Abstract

OBJECTIVE:

To investigate the influence of the vascular endothelial growth factor( VEGF) antibody targeted vascular therapy on the expression of human collagen type I in hyperplastic scar of nude mice.

METHODS:

The hyperplastic scar from one female burn patient with 1% TBSA deep-partial thickness burns were implanted into subcutaneous skin of scapular region of 48 nude mice. Three weeks later, the nude mice were divide into large dose (LA) , medium dose (MD) , small dose (SD) and control groups, with 12 mice in each group. The mice in LA,MD and SD groups were injected with 200 microl of 15,10, 5 microg/ml VEGF monoclonal antibody diluted in 0.01 mol/L PBS, respectively in the scar twice a week for 3 weeks, while those in C group were injected with equal amount of 0. 01 mol/L PBS. The area and volume of the scar in each group were calculated and histological changes were observed, and the expression of collagen type I mRNA and its protein in each group were determined 3 days after treatment.

RESULTS:

The volume of scar in LA, MD, SD and C groups were (55.3 +/-4.1, 67.9 +/-5.7, 78.9 +/-5.5, 85.0 +7.3) mm(3), respectively. Compared with that in C group, the volume of the scar were significantly decreased in AD and MD groups ( P <0.05). A few number of vessels and fibroblasts were observed in LD, MD groups, with decreased number of collagen fibers arranged in order. Compared with that in C group ,The expression of procollagen type I mRNA and its protein in C group was obviously higher than those in LD and MD groups ( P < 0. 05) , but it was similar to those in SD group.

CONCLUSION:

VEGF targeted vascular therapy is beneficial for the inhibition of the angiopoietins of hyperplastic scar, the expression of collagen , and the growth of scar.

• Authors: Yi-gang Yue, Chang-wen Jiang, Pei-ying Li, Si Zhou
• Journal: Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns (Zhonghua Shao Shang Za Zhi) Vol. 22 Issue 6 Pg. 427-30 (Dec 2006) ISSN: 1009-2587 [Print] China
• PMID: 17438687 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Collagen Type I
• RNA, Messenger
• VEGFA protein, human
• Vascular Endothelial Growth Factor A

Topics

• Adult
• Animals
• Antibodies, Monoclonal (therapeutic use)
• Cicatrix, Hypertrophic (metabolism, therapy)
• Collagen Type I (metabolism)
• Disease Models, Animal
• Female
• Gene Expression
• Humans
• Hyperplasia (metabolism, therapy)
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Neovascularization, Pathologic (metabolism)
• RNA, Messenger (metabolism)
• Vascular Endothelial Growth Factor A (immunology)