Abstract | PURPOSE: MATERIALS AND METHODS: A total of 30 human fetal testes from abortuses at a gestational age of 10 to 40 weeks, 33 human seminomas and 11 human Leydig cell tumors were retrieved from the archives of the departments of pathology. Immunohistochemistry was performed with affinity purified p44 and IgG purified protein arginine methyltransferase 5 polyclonal antibodies. RESULTS:
Protein arginine methyltransferase 5 and p44 were expressed predominantly as nuclear proteins in fetal Leydig cells and human adult nonneoplastic testes, including germ cells and Leydig cells, while they were expressed in the cytoplasm of germ cells of the fetal testis. Expression was strongest in the fetal testis during the second trimester. Compared to adult nonneoplastic testes, human seminoma and Leydig tumor cells showed a marked decrease in nuclear expression of p44 and protein arginine methyltransferase 5 with a concomitant marked increase in cytoplasmic expression of these proteins. Furthermore, average testicular size was increased by 29% in p44(+/-) heterzygotic mice. CONCLUSIONS:
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Authors | John J Liang, Zhengxin Wang, Luis Chiriboga, M Alba Greco, Ellen Shapiro, Hongying Huang, Ximing J Yang, Jiaoti Huang, Yi Peng, Jonathan Melamed, Michael J Garabedian, Peng Lee |
Journal | The Journal of urology
(J Urol)
Vol. 177
Issue 5
Pg. 1918-22
(May 2007)
ISSN: 0022-5347 [Print] United States |
PMID | 17437848
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- RNA, Messenger
- Transcription Factors
- WDR77 protein, human
- Protein Methyltransferases
- PRMT5 protein, human
- Protein-Arginine N-Methyltransferases
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Topics |
- Adult
- Animals
- Blotting, Northern
- Female
- Gene Expression Regulation, Developmental
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Leydig Cell Tumor
(metabolism, pathology)
- Male
- Mice
- Mice, Inbred C57BL
- Protein Methyltransferases
(genetics, metabolism)
- Protein-Arginine N-Methyltransferases
- RNA, Messenger
(genetics)
- Seminoma
(metabolism, pathology)
- Testicular Neoplasms
(metabolism, pathology)
- Testis
(embryology, metabolism)
- Transcription Factors
(genetics, metabolism)
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