HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

[Confocal-microscopic study of skeletal muscle fibre membrane organelles during Zenker's (spreading) necrosis].

Abstract
The changes of T-system and cellular acidic organelles during spreading (Zenker's) necrosis of frog skeletal muscle fibres have been investigated using laser confocal microscopy and several vital fluorescent dyes acridine orange, RH 414, DiOC6(3), rhodamine 123, fluorescein dextran. The formation of numerous vacuoles as a result of local T-system swelling is most characteristic for initial steps of Zenker's necrosis. Vacuoles can attain tens microns in length. They are located both near nuclear poles and between myofibres. Vacuoles maintain connections with the extracellular space up to the moment of contraction knot rejection, and under definite conditions (glycerol influx to fibre) vacuoles are reversible. They deform nuclei and sarcoplasmic reticulum cisternae. Cellular acidic organelles, accumulating acridine orange (lysosomes, late endosomes, Golgi apparatus cisternae) are situated in direct vicinity with normal and vacuolated T-system. The increase in acidic organelles number and size occur during the pathological process development, and tendency to vacuoles clusterization may be seen. Vacuolation of T-system during necrosis is not followed by vacuole content acidification. The role of cellular acidic organelles and of T-system vacuolation in the development of different muscle pathological changes is discussed.
AuthorsS A Krolenko, S Ia Adamian, T N Beliaeva, T P Mozhenok, A V Salova
JournalTsitologiia (Tsitologiia) Vol. 49 Issue 2 Pg. 107-14 ( 2007) ISSN: 0041-3771 [Print] Russia (Federation)
PMID17432595 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Fluorescent Dyes
Topics
  • Animals
  • Cell Nucleus (pathology)
  • Fluorescent Dyes
  • Membranes (pathology)
  • Microscopy, Confocal
  • Muscle Fibers, Skeletal (pathology)
  • Muscle, Skeletal (pathology)
  • Muscular Diseases (pathology)
  • Necrosis (pathology)
  • Organelles (pathology)
  • Rana temporaria
  • Vacuoles (pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: