Inhibition of severe acute respiratory syndrome-associated coronavirus infection by equine neutralizing antibody in golden Syrian hamsters.

Abstract

Equine anti-severe acute respiratory syndrome-associated coronavirus F(ab')(2) has been verified to protect mice from infection with severe acute respiratory syndrome-associated coronavirus (SARS-CoV). However, before potential clinical application, the antibody needs to be tested in as many animal models as possible to ensure its safety and efficiency. In this study, after verification by various methods that the golden Syrian hamster constitutes a model susceptible to SARS-CoV infection, we confirmed that the antibody could protect animals completely from SARS-CoV infection in the preventive setting. More importantly, the antibody could reduce viral titers or copies by approximately 10(3)- to 10(4)-fold in animal lung after virus exposure, compared with negative control. These data provide further evidence to warrant clinical studies of this antibody in the treatment and prevention of SARS.

• Authors: Guangyu Zhao, Bing Ni, Haiyan Jiang, Deyan Luo, Marek Pacal, Lili Zhou, Liangyan Zhang, Li Xing, Liangyan Zhang, Zhengcai Jia, Zhihua Lin, Li Wang, Jintao Li, Yunfei Liang, Xinfu Shi, Tingting Zhao, Liyun Zhou, Yuzhang Wu, Xiliang Wang
• Journal: Viral immunology (Viral Immunol) Vol. 20 Issue 1 Pg. 197-205 ( 2007) ISSN: 0882-8245 [Print] United States
• PMID: 17425434 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Immunoglobulin Fab Fragments

Topics

• Administration, Intranasal
• Animals
• Antibodies, Viral (immunology)
• Cricetinae
• Horses
• Immunoglobulin Fab Fragments (immunology)
• Mesocricetus
• Neutralization Tests
• Polymerase Chain Reaction
• Severe Acute Respiratory Syndrome (prevention & control)