Inhibition of bone resorption in cultures of mouse calvariae by apicularen A.

Abstract

Apicularens A and B were isolated from the myxobacterial genus Chondromyces apiculatus JW184. Apicularen A inhibited bafilomycin A1-sensitive ATP-dependent proton transport into microsome vesicles more potently than apicularen B. Bone resorption in cultures of mouse calvariae induced by human parathyroid hormone (PTH) or interleukin-1beta (IL-1beta) was inhibited by apicularen A at 10 and 100 nM, while apicularen B had no effect. The bisphosphonate incadronate inhibited bone resorption at 100 nM, being less effective than apicularen A. Our findings indicate that apicularen A inhibits bone resorption induced by PTH or IL-1beta more potently than apicularen B, probably due to inhibition of the V-ATPase.

• Authors: JangJa Hong, Hirokazu Sasaki, Kazuaki Niikura, Maiko Yanai, Yasuhiro Nakano, Aya Yokomakura, Kenji Ishihara, Noriyasu Hirasawa, Young-Sook Kang, Joa Sub Oh, Jong Hwan Kwak, OkPyo Zee, Kazuo Ohuchi
• Journal: Planta medica (Planta Med) Vol. 73 Issue 2 Pg. 173-5 (Feb 2007) ISSN: 0032-0943 [Print] Germany
• PMID: 17415879 (Publication Type: Journal Article)

Chemical References

• Bridged Bicyclo Compounds, Heterocyclic
• Diphosphonates
• Macrolides
• Parathyroid Hormone
• apicularen A
• apicularen B
• cimadronate
• bafilomycin A1
• Vacuolar Proton-Translocating ATPases

Topics

• Animals
• Bone Resorption
• Bridged Bicyclo Compounds, Heterocyclic (chemistry, isolation & purification, pharmacology)
• Diphosphonates (chemistry, pharmacology)
• Humans
• Macrolides (chemistry, isolation & purification, pharmacology)
• Mice
• Myxococcales (chemistry)
• Parathyroid Hormone (pharmacology)
• Skull (drug effects)
• Vacuolar Proton-Translocating ATPases (antagonists & inhibitors)