Abstract |
We report on the storage of platelet concentrates and the effects on in vivo and in vitro platelet function tests of adding the platelet inhibitors prostaglandin E1 and theophylline to a plasma-free synthetic medium and storage in containers with reduced surface-to-volume ratio. Paired in vivo studies on platelets labeled with indium 111 after 14-day storage demonstrated higher recoveries (mean +/- SD) of 23% +/- 9% and longer survivals of 109 +/- 59 hours for the test group versus 8% +/- 10% and 19 +/- 22 hours, respectively (p less than 0.01), for the control group (synthetic medium with no inhibitors and use of standard containers). The improved viability was associated with a significantly lower glycolytic rate; better maintenance of other in vitro parameters including respiratory activity, adenosine triphosphate levels, hypotonic shock response, surface glycoprotein Ib (by flow cytometry); and improved preservation of morphologic integrity (p less than 0.05). In particular, a strong inverse correlation (r = -0.91) was observed between in vivo recoveries and the fraction of platelets negative for glycoprotein Ib. We conclude that avoidance of platelet activation by a combination of inhibitors, plasma removal, and reduced container surface improves platelet respiratory activity, adenosine triphosphate and glycoprotein Ib levels, and posttransfusion viability of platelet concentrates stored for 14 days.
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Authors | S Holme, A Bode, W A Heaton, S Sawyer |
Journal | The Journal of laboratory and clinical medicine
(J Lab Clin Med)
Vol. 119
Issue 2
Pg. 144-50
(Feb 1992)
ISSN: 0022-2143 [Print] United States |
PMID | 1740627
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Culture Media, Serum-Free
- Platelet Membrane Glycoproteins
- Adenosine Triphosphate
- Theophylline
- Alprostadil
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Topics |
- Adenosine Triphosphate
(analysis)
- Alprostadil
(analysis, pharmacology)
- Blood Platelets
(chemistry, cytology, physiology)
- Blood Preservation
(methods)
- Cell Survival
(drug effects, physiology)
- Culture Media, Serum-Free
(analysis, pharmacology)
- Energy Metabolism
(drug effects)
- Flow Cytometry
- Humans
- Oxygen Consumption
(physiology)
- Platelet Membrane Glycoproteins
(analysis)
- Theophylline
(analysis, pharmacology)
- Time Factors
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