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Metallopeptidase, neurolysin, as a novel molecular tool for analysis of properties of cancer-producing matrix metalloproteinases-2 and -9.

Abstract
To compare the substrate preferences of rat brain neurolysin and cancer-producing matrix metalloproteinases (MMPs), which have the same architecture in their catalytic domains, the cleavage activity of neurolysin toward MMP-specific fluorescence-quenching peptides was quantitatively measured. The results show that neurolysin effectively cleaved MOCAc [(7-methoxy coumarin-4-yl) acetyl]-RPKPYANvaWMK(Dnp[2,4-dinitrophenyl])-NH(2), a specific substrate of MMP-2 and MMP-9, but hardly cleaved MOCAc-RPKPVENvaWRK(Dnp)-NH(2), a specific substrate of MMP-3, suggesting that neurolysin has a similar substrate preference to MMP-2 and MMP-9. A structural comparison between neurolysin and MMP-9 showed the similar key amino acid residues for substrate recognition. The possible application of neurolysin displayed on the yeast cell surface, as a safe protein alternative to MMP-2 and MMP-9 which induce cancer cell growth, invasion, and metastasis, to analysis of properties of the MMPs, including the screening of inhibitors and analysis of inhibition mechanism etc., are also discussed.
AuthorsTetsuya Kadonosono, Michiko Kato, Mitsuyoshi Ueda
JournalApplied microbiology and biotechnology (Appl Microbiol Biotechnol) Vol. 75 Issue 6 Pg. 1285-91 (Jul 2007) ISSN: 0175-7598 [Print] Germany
PMID17404728 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Metalloendopeptidases
  • neurolysin
  • Matrix Metalloproteinase 2
  • Mmp2 protein, rat
  • Matrix Metalloproteinase 9
Topics
  • Animals
  • Matrix Metalloproteinase 2 (chemistry, physiology)
  • Matrix Metalloproteinase 9 (chemistry, physiology)
  • Metalloendopeptidases (chemistry, physiology)
  • Neoplasms (enzymology)
  • Rats
  • Substrate Specificity

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