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Effects of acetylpuerarin on hippocampal neurons and intracellular free calcium subjected to oxygen-glucose deprivation/reperfusion in primary culture.

AbstractOBJECTIVES:
This study was undertaken to find out the effects of acetylpuerarin on hippocampal neurons and intracellular free calcium in primary culture subjected to oxygen-glucose deprivation/reperfusion.
METHODS:
According to different reperfusion time (1 h, 6 h, 12 h, 24 h), three concentrations (1.6 micromol l(-1), 0.4 micromol l(-1), 0.1 micromol l(-1)) of acetylpuerarin, and MK-801 (10 micromol l(-1)), a positive control drug, neurons were randomly divided into 21 groups. Each group was observed by inverted phase contrast microscope; neuron viability was measured by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT); intracellular Ca(2+) was observed by Fura-2/AM ester through fluorospectrophotometer.
RESULTS:
The injured neurons were protected and degeneration and necrosis were alleviated in treatment groups of acetylpuerarin and MK-801. Acetylpuerarin increased the neuron viability at high, middle and low concentrations. Fluorescence detection results showed that the calcium concentration in the group treated with acetylpuerarin and MK-801 was lowered in each reperfusion time.
CONCLUSION:
Our results demonstrated that acetylpuerarin could protect the hippocampal neurons from ischemia-reperfusion injury in rats by alleviating the morphological damage, increasing neuron viability and decreasing calcium concentration in neuron.
AuthorsRui Liu, Xin-bing Wei, Xiu-Mei Zhang
JournalBrain research (Brain Res) Vol. 1147 Pg. 95-104 (May 25 2007) ISSN: 0006-8993 [Print] Netherlands
PMID17397811 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Isoflavones
  • Neuroprotective Agents
  • acetylpuerarin
  • Glucose
  • Calcium
Topics
  • Analysis of Variance
  • Animals
  • Calcium (metabolism)
  • Cell Hypoxia (drug effects)
  • Cell Survival (drug effects)
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Glucose (deficiency)
  • Hippocampus (cytology, drug effects, metabolism)
  • Intracellular Fluid (drug effects, metabolism)
  • Ischemia (drug therapy, pathology)
  • Isoflavones (pharmacology)
  • Neurons (cytology, drug effects, metabolism)
  • Neuroprotective Agents (pharmacology)
  • Random Allocation
  • Rats
  • Rats, Wistar
  • Reperfusion Injury (drug therapy, pathology)
  • Time Factors

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