Abstract | OBJECTIVES: This study was undertaken to find out the effects of acetylpuerarin on hippocampal neurons and intracellular free calcium in primary culture subjected to oxygen- glucose deprivation/reperfusion. METHODS: According to different reperfusion time (1 h, 6 h, 12 h, 24 h), three concentrations (1.6 micromol l(-1), 0.4 micromol l(-1), 0.1 micromol l(-1)) of acetylpuerarin, and MK-801 (10 micromol l(-1)), a positive control drug, neurons were randomly divided into 21 groups. Each group was observed by inverted phase contrast microscope; neuron viability was measured by the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT); intracellular Ca(2+) was observed by Fura-2/AM ester through fluorospectrophotometer. RESULTS: The injured neurons were protected and degeneration and necrosis were alleviated in treatment groups of acetylpuerarin and MK-801. Acetylpuerarin increased the neuron viability at high, middle and low concentrations. Fluorescence detection results showed that the calcium concentration in the group treated with acetylpuerarin and MK-801 was lowered in each reperfusion time. CONCLUSION: Our results demonstrated that acetylpuerarin could protect the hippocampal neurons from ischemia-reperfusion injury in rats by alleviating the morphological damage, increasing neuron viability and decreasing calcium concentration in neuron.
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Authors | Rui Liu, Xin-bing Wei, Xiu-Mei Zhang |
Journal | Brain research
(Brain Res)
Vol. 1147
Pg. 95-104
(May 25 2007)
ISSN: 0006-8993 [Print] Netherlands |
PMID | 17397811
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Isoflavones
- Neuroprotective Agents
- acetylpuerarin
- Glucose
- Calcium
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Topics |
- Analysis of Variance
- Animals
- Calcium
(metabolism)
- Cell Hypoxia
(drug effects)
- Cell Survival
(drug effects)
- Cells, Cultured
- Dose-Response Relationship, Drug
- Glucose
(deficiency)
- Hippocampus
(cytology, drug effects, metabolism)
- Intracellular Fluid
(drug effects, metabolism)
- Ischemia
(drug therapy, pathology)
- Isoflavones
(pharmacology)
- Neurons
(cytology, drug effects, metabolism)
- Neuroprotective Agents
(pharmacology)
- Random Allocation
- Rats
- Rats, Wistar
- Reperfusion Injury
(drug therapy, pathology)
- Time Factors
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