Abstract | OBJECTIVE: METHODS: More than 2,500 sera from patients with connective tissue disease (including myositis and ILD) and controls were examined for anti-AsnRS autoantibodies by immunoprecipitation (IP). Positive and control sera were tested for the ability to inhibit AsnRS by preincubation of the enzyme source with the serum. The HLA class II (DRB1, DQA1, DQB1, DPB1) alleles were identified from restriction fragment length polymorphism of polymerase chain reaction-amplified genomic DNA. RESULTS: Anti-AsnRS antibodies were identified in the sera of 8 patients (5 Japanese, 1 American, 1 German, and 1 Korean) by IP of the same distinctive set of tRNA and protein that differed from those precipitated by the other 5 antisynthetases, and these antibodies showed specific inhibition of AsnRS activity. Two of these patients had DM, but 7 of 8 (88%) had ILD. Four patients (50%) had arthritis, and 1 had Raynaud's phenomenon. This antisynthetase was very rare among myositis patients (present in 0% of Japanese myositis patients), but it was found in 3% of Japanese ILD patients. Thus, most patients with anti-AsnRS had chronic ILD with or without features of connective tissue disease. Interestingly, all 4 Japanese patients tested had DR2 (DRB1*1501/1502), compared with 33% of healthy controls. CONCLUSION:
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Authors | Michito Hirakata, Akira Suwa, Tetsuya Takada, Shinji Sato, Sonoko Nagai, Ekkehard Genth, Yeong W Song, Tsuneyo Mimori, Ira N Targoff |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 56
Issue 4
Pg. 1295-303
(Apr 2007)
ISSN: 0004-3591 [Print] United States |
PMID | 17393393
(Publication Type: Case Reports, Journal Article, Multicenter Study, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Autoantibodies
- Autoantigens
- HLA-DR2 Antigen
- RNA, Transfer, Amino Acyl
- Aspartate-tRNA Ligase
- asparaginyl-tRNA synthetase
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Topics |
- Aged
- Arthritis
(genetics, immunology, pathology)
- Aspartate-tRNA Ligase
(immunology)
- Autoantibodies
(blood)
- Autoantigens
(immunology)
- Dermatomyositis
(genetics, immunology, pathology)
- Fatal Outcome
- Female
- HLA-DR2 Antigen
(genetics)
- Haplotypes
- HeLa Cells
- Histocompatibility Testing
- Humans
- Lung Diseases, Interstitial
(genetics, immunology, pathology)
- Male
- Middle Aged
- Polymorphism, Restriction Fragment Length
- Polymyositis
(genetics, immunology, pathology)
- RNA, Transfer, Amino Acyl
(immunology)
- Syndrome
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