HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Depletion of mitochondrial DNA by ethidium bromide treatment inhibits the proliferation and tumorigenesis of T47D human breast cancer cells.

Abstract
In order to investigate the role of mitochondrial DNA (mtDNA) in human breast cancer cell proliferation and apoptosis, a mtDNA-deficient cell line, T47D rho(0), was generated following a long-term exposure to ethidium bromide (EtBr). T47D rho(0) cells showed a marked decrease in mitochondrial membrane potential (DeltaPsi(m)). However, the apoptosis rate of T47D rho(0) cells was the same as that of their parental cells, suggesting that the change in DeltaPsi(m) was insufficient to induce cell death. Electromicroscopy revealed a profound alteration of mitochondrial morphology, which was consistent with the loss of mtDNA and the decrease in DeltaPsi(m). Disruption of mtDNA resulted in a slower proliferation rate in tissue culture and a reduction in anchorage-independent growth. An in vivo assay revealed a severe impairment of tumorigenicity in T47D rho(0) cells, indicating the biological relevance of in vitro studies. Taken together, our results suggest that the integrity of mtDNA plays a critical role in human breast cancer cell proliferation and tumorigenesis.
AuthorsMan Yu, Yurong Shi, Xiyin Wei, Yi Yang, Yunli Zhou, Xishan Hao, Ning Zhang, Ruifang Niu
JournalToxicology letters (Toxicol Lett) Vol. 170 Issue 1 Pg. 83-93 (Apr 05 2007) ISSN: 0378-4274 [Print] Netherlands
PMID17391873 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA, Mitochondrial
  • Ethidium
Topics
  • Animals
  • Apoptosis (drug effects)
  • Breast Neoplasms (pathology)
  • Cell Line, Tumor
  • Cell Proliferation (drug effects)
  • DNA, Mitochondrial (drug effects, metabolism, ultrastructure)
  • Ethidium (pharmacology)
  • Female
  • Humans
  • Membrane Potential, Mitochondrial (drug effects)
  • Mice
  • Microscopy, Electron, Transmission
  • Mitochondrial Membranes (drug effects, metabolism, ultrastructure)
  • Neoplasm Transplantation
  • Neoplasms, Experimental (metabolism, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: