Effects of KNK437 on heat-induced methylation of histone H3 in human oral squamous cell carcinoma cells.

Abstract

PURPOSE:

Methylation of H3 at lysine 4 (H3 Lys4) may be correlated with active gene trascription, whereas methylation of H3 at lysine 9 (H3 Lys9) may be linked to gene repression in murine cells and Schizosaccharomyces pombe.

METHODS:

Using Western blot analysis, heat-induced changes were studied in two human oral cancer cell lines, HSC4 (thermoresistant cells) and KB (thermosensitive cells). Histone H3 changes were studied; in particular for H3-Lys4 and H3-Lys9 methylation combined with KNK437.

RESULTS:

Heating of HSC4 cells at 45 degrees C for 20 min and KB cells for 3 min gradually increased H3-Lys4 and H3-Lys9 methylation. Treatment of both cells with 100 microM KNK437 before or after heat-treatment inhibited methylation of H3-Lys4, while methylation of H3 Lys9 remained unaffected. Use of KNK437 either before or after heat treatment inhibited the expression of HSP70.

CONCLUSIONS:

The findings suggest that heat-induced methylation of histone H3 may be correlated with the induction of HSPs by heating.

• Authors: Keiji Matsuda, Shin-Ya Nakagawa, Tomoya Nakano, Jun-Ichi Asaumi, Ganesh Chandra Jagetia, Shoji Kawasaki
• Journal: International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group (Int J Hyperthermia) Vol. 22 Issue 8 Pg. 729-35 (Dec 2006) ISSN: 0265-6736 [Print] England
• PMID: 17391001 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Benzhydryl Compounds
• Heat-Shock Proteins
• Histones
• KNK 437
• Pyrrolidinones

Topics

• Benzhydryl Compounds (pharmacology)
• Cell Line, Tumor
• Heat-Shock Proteins (antagonists & inhibitors)
• Histones (chemistry, drug effects)
• Humans
• Hyperthermia, Induced (adverse effects)
• KB Cells
• Methylation
• Mouth Neoplasms (therapy)
• Neoplasms, Squamous Cell (therapy)
• Pyrrolidinones (pharmacology)