Plasma resistin, associated with single nucleotide polymorphism -420, is correlated with insulin resistance, lower HDL cholesterol, and high-sensitivity C-reactive protein in the Japanese general population.

Abstract

OBJECTIVE:

Resistin, secreted from adipocytes, causes insulin resistance in rodents. We previously reported that the G/G genotype of a resistin gene promoter single nucleotide polymorphism (SNP) at -420 increases type 2 diabetes susceptibility by enhancing promoter activity. We report here on the relation between plasma resistin and either SNP -420 genotype or factors related to insulin resistance.

RESEARCH DESIGN AND METHODS:

We cross-sectionally analyzed 2,078 community-dwelling Japanese subjects attending a yearly medical checkup. The SNP -420 genotype was determined by TaqMan analysis. Fasting plasma resistin was measured using an enzyme-linked immunosorbent assay kit.

RESULTS:

Plasma resistin was associated with the SNP -420 genotype (P < 0.0001), which was highest in G/G followed by C/G and C/C. Plasma resistin was higher in elderly individuals, female subjects, nondrinkers, and subjects with high blood pressure (P < 0.001, 0.003, <0.001, and 0.001, respectively). Simple regression analysis revealed that age, female sex, homeostasis model assessment of insulin resistance (HOMA-IR) index, systolic blood pressure, low HDL cholesterol, and high-sensitivity C-reactive protein (hs-CRP) were positively correlated with plasma resistin (P < 0.001, 0.003, <0.001, 0.004, <0.001, and 0.003, respectively). Multiple regression analysis adjusted for age, sex, and BMI revealed that plasma resistin was an independent factor for HOMA-IR, low HDL cholesterol, and hs-CRP (P = 0.001, <0.001, and 0.006, respectively).

CONCLUSIONS:

Plasma resistin was associated with SNP -420 and was correlated with insulin resistance, low serum HDL cholesterol, and high hs-CRP in the Japanese general population.

• Authors: Haruhiko Osawa, Yasuharu Tabara, Ryuichi Kawamoto, Jun Ohashi, Masaaki Ochi, Hiroshi Onuma, Wataru Nishida, Kazuya Yamada, Jun Nakura, Katsuhiko Kohara, Tetsuro Miki, Hideichi Makino
• Journal: Diabetes care (Diabetes Care) Vol. 30 Issue 6 Pg. 1501-6 (Jun 2007) ISSN: 1935-5548 [Electronic] United States
• PMID: 17384338 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cholesterol, LDL
• Glycated Hemoglobin A
• Insulin
• Resistin
• C-Reactive Protein

Topics

• Aged
• Blood Pressure
• C-Reactive Protein (metabolism)
• Cholesterol, LDL (blood)
• Cross-Sectional Studies
• Female
• Genotype
• Glycated Hemoglobin (analysis)
• Humans
• Insulin (blood)
• Insulin Resistance (genetics)
• Japan
• Male
• Middle Aged
• Polymorphism, Single Nucleotide
• Reference Values
• Resistin (blood)