Abstract | BACKGROUND: METHODS: Marrow stromal cells were expanded in vitro and prelabeled with bromodeoxyuridine. Spinal cord ischemia was induced in rabbits by infrarenal aortic occlusion for 30 minutes. Four groups were enrolled. About 1 x 10(8) MSCs were intrathecally injected 2 hours (group MSC-2h), 24 hours (group MSC-24h), or 48 hours (group MSC-48h) after spinal cord ischemia, respectively. The control group received intrathecal injection of medium alone. Hind-limb motor function was assessed during a 28-day recovery period with Tarlov criteria, and then histologic examination was performed. RESULTS: Marrow stromal cells still could be found in the spinal cord 4 weeks after transplantation. The capillary density in the ventral gray matter was significantly increased in the three MSC-treated groups (p < 0.01 versus control group, respectively). After a 28-day recovery, marked functional improvement was detected in group MSC-2h (from day 1 to 28, p < 0.05, versus control group, respectively) and group MSC-24h (from day 14 to 28, p < 0.05, versus control group, respectively), but not in group MSC-48h. The number of intact motor neurons was much greater in group MSC-2h (p < 0.05, versus control group). CONCLUSIONS:
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Authors | Enyi Shi, Teruhisa Kazui, Xiaojing Jiang, Naoki Washiyama, Katsushi Yamashita, Hitoshi Terada, Abul Hasan Muhammad Bashar |
Journal | The Annals of thoracic surgery
(Ann Thorac Surg)
Vol. 83
Issue 4
Pg. 1484-90
(Apr 2007)
ISSN: 1552-6259 [Electronic] Netherlands |
PMID | 17383362
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Biopsy, Needle
- Bone Marrow Transplantation
(methods)
- Disease Models, Animal
- Immunohistochemistry
- Injections, Spinal
- Neovascularization, Physiologic
- Neurologic Examination
- Rabbits
- Random Allocation
- Recovery of Function
- Reference Values
- Sensitivity and Specificity
- Spinal Cord Injuries
(pathology, therapy)
- Spinal Cord Ischemia
(pathology, therapy)
- Stromal Cells
(transplantation)
- Transplantation, Autologous
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