| Abstract | S(IV) (SO(2),HSO(3)(-)andSO(3)(2-)) autoxidation catalyzed by Cu(II)/tetraglycine complexes in the presence of DNA or 2'-deoxyguanosine (dGuo) resulted in DNA strand breaks and formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), respectively. Ni(II), Co(II) or Mn(II) (1.0x10(-4)M) complexes had much smaller effects. Cu(II)/tetraglycine (1.0x10(-4)M) in the presence of Ni(II) or Mn(II) (10(-7)-10(-6)M) and S(IV) showed remarkable synergistic effect with these metal ions producing a higher yield of 8-oxodGuo. Oxidation of dGuo and DNA damage were attributed to oxysulfur radicals formed as intermediates in S(IV) autoxidation catalyzed by transition metal ions. SO*(3)(-) and HO* radicals were detected by EPR-spin trapping experiments with DMPO (5,5-dimethyl-1-pyrroline-N-oxide). |
| Authors | Ruben G M Moreno, María V Alipázaga, Osmar F Gomes, Edlaine Linares, Marisa H G Medeiros, Nina Coichev
(Affiliation: Instituto de Química, Universidade de São Paulo, CP 26077, CEP 05513-970, São Paulo, SP, Brazil.)
|
| Journal | Journal of inorganic biochemistry
(J Inorg Biochem)
Vol. 101
Issue 5
Pg. 866-75
(May 2007)
ISSN: 0162-0134 United States |
| PMID | 17383005
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Oligopeptides
- Organometallic Compounds
- glycyl-glycyl-glycyl-glycine
- Copper
- Sulfur Dioxide
- Deoxyguanosine
|
| Topics |
- Copper
(chemistry)
- DNA Damage
- Deoxyguanosine
(chemistry)
- Electron Spin Resonance Spectroscopy
- Electrophoresis, Agar Gel
- Oligopeptides
(chemistry)
- Organometallic Compounds
(chemistry)
- Oxidation-Reduction
- Spectrophotometry
- Sulfur Dioxide
(chemistry)
|
