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Toxoplasma gondii: DNA vaccination with genes encoding antigens MIC2, M2AP, AMA1 and BAG1 and evaluation of their immunogenic potential.

Abstract
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-gamma synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes.
AuthorsGeorge Dautu, Biscah Munyaka, Gabriella Carmen, Guohong Zhang, Yoshitaka Omata, Xuan Xuenan, Makoto Igarashi
JournalExperimental parasitology (Exp Parasitol) Vol. 116 Issue 3 Pg. 273-82 (Jul 2007) ISSN: 0014-4894 [Print] United States
PMID17379212 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • AMA1 protein, Toxoplasma gondii
  • Antibodies, Protozoan
  • Antigens, Protozoan
  • BAG1 protein, Toxoplasma
  • Heat-Shock Proteins
  • Immunoglobulin G
  • MIC2 protein, Toxoplasma gondii
  • Membrane Proteins
  • Protozoan Proteins
  • Protozoan Vaccines
  • Recombinant Proteins
  • TgM2AP protein, Toxoplasma gondii
  • Vaccines, DNA
  • Interferon-gamma
Topics
  • Animals
  • Antibodies, Protozoan (biosynthesis, blood)
  • Antigens, Protozoan (genetics, immunology)
  • Biolistics
  • Cell Line
  • Female
  • Heat-Shock Proteins (genetics, immunology)
  • Humans
  • Immunity, Cellular
  • Immunoglobulin G (biosynthesis, blood)
  • Interferon-gamma (biosynthesis)
  • Membrane Proteins (genetics, immunology)
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Protozoan Proteins (genetics, immunology)
  • Protozoan Vaccines (administration & dosage, immunology)
  • Recombinant Proteins (genetics, immunology)
  • Spleen (cytology, immunology)
  • Toxoplasma (genetics, immunology)
  • Toxoplasmosis, Animal (prevention & control)
  • Vaccines, DNA (administration & dosage, immunology)

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