Abstract |
A combination of antigenic regions of microneme proteins have been previously reported as being protective against chronic toxoplasmosis. In this work, we evaluated immune responses induced by immunizing BALB/c and C57BL/6 mice intradermally with plasmid DNA encoding the protein sequences of Toxoplasma gondii AMA1, MIC2, M2AP and BAG1. Mice immunized with the AMA1 gene developed high levels of serum IgG2a and c antibodies as well as cellular immune responses associated with IFN-gamma synthesis suggesting a modulated Th1 type of response. Immunization with the AMA1 gene resulted in a partial but significant protection against the acute phase of toxoplasmosis compared to MIC2, M2AP and BAG1 genes. Therefore, the AMA1 gene appears to generate a strong specific immune response and also provides effective protection against toxoplasmosis more than the MIC2, M2AP and BAG1 genes.
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Authors | George Dautu, Biscah Munyaka, Gabriella Carmen, Guohong Zhang, Yoshitaka Omata, Xuan Xuenan, Makoto Igarashi |
Journal | Experimental parasitology
(Exp Parasitol)
Vol. 116
Issue 3
Pg. 273-82
(Jul 2007)
ISSN: 0014-4894 [Print] United States |
PMID | 17379212
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- AMA1 protein, Toxoplasma gondii
- Antibodies, Protozoan
- Antigens, Protozoan
- BAG1 protein, Toxoplasma
- Heat-Shock Proteins
- Immunoglobulin G
- MIC2 protein, Toxoplasma gondii
- Membrane Proteins
- Protozoan Proteins
- Protozoan Vaccines
- Recombinant Proteins
- TgM2AP protein, Toxoplasma gondii
- Vaccines, DNA
- Interferon-gamma
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Topics |
- Animals
- Antibodies, Protozoan
(biosynthesis, blood)
- Antigens, Protozoan
(genetics, immunology)
- Biolistics
- Cell Line
- Female
- Heat-Shock Proteins
(genetics, immunology)
- Humans
- Immunity, Cellular
- Immunoglobulin G
(biosynthesis, blood)
- Interferon-gamma
(biosynthesis)
- Membrane Proteins
(genetics, immunology)
- Mice
- Mice, Inbred BALB C
- Mice, Inbred C57BL
- Protozoan Proteins
(genetics, immunology)
- Protozoan Vaccines
(administration & dosage, immunology)
- Recombinant Proteins
(genetics, immunology)
- Spleen
(cytology, immunology)
- Toxoplasma
(genetics, immunology)
- Toxoplasmosis, Animal
(prevention & control)
- Vaccines, DNA
(administration & dosage, immunology)
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