[Effects of Genistein on the proliferation and expression of survivin in salivary adenoid cystic carcinoma cell line SACC-83].

Abstract

OBJECTIVE:

To investigate the anti-proliferation effect of tyrosine protein kinase inhibitor, Genistein, on human salivary adenoid cystic carcinoma cell line SACC-83, and its effect on Survivin expression.

METHODS:

SACC-83 cells were treated with different concentration Genistein for different time, cell survival rate was calculated with MTT assay, apoptosis was detected with flow cytometry, the expression of Survivin was quantitatively analyzed by Western blotting and FluorChem V2.0 software.

RESULTS:

When treated with Genistein of certain concentration for certain time, SACC-83 cell growth was significantly inhibited. With the increase of concentration and elongation of acting time, the inhibitory effects increase. Treated with 220 micromol/L Genistein for 72 hours, SACC-83 cell growth was significantly inhibited, cell apoptosis was induced (P < 0.01), and the expression of Survivin decreased.

CONCLUSION:

Genistein inhibits the growth of human salivary adenoid cystic carcinoma cell line SACC-83, and induces cell apoptosis; the decrease of Survivin expression may be one of the mechanisms of Genistein inducing apoptosis.

• Authors: Jie Ma, Zhi-hong Zong, Zhao-yuan Wang, Ming Zhong
• Journal: Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology (Hua Xi Kou Qiang Yi Xue Za Zhi) Vol. 25 Issue 1 Pg. 97-9 (Feb 2007) ISSN: 1000-1182 [Print] China
• PMID: 17375591 (Publication Type: Journal Article)

Chemical References

• Genistein

Topics

• Apoptosis
• Carcinoma, Adenoid Cystic
• Cell Line
• Cell Line, Tumor
• Cell Proliferation
• Genistein
• Humans
• Salivary Gland Neoplasms