Alpha1,2fucosylation is a superior predictor of postoperative prognosis for colorectal cancer compared with blood group A, B, or sialyl Lewis X antigen generated within colorectal tumor tissues.

Abstract

BACKGROUND:

We have previously demonstrated tumor-specific alpha1,2fucosylation, which is associated with resistance of tumor cells to anticancer treatment in human colorectal tumor tissues. By using the YB-2 monoclonal antibody, the resulting products have been identified as Y, Le(b), and H type 2 antigens in colorectal tumor tissues.

METHODS:

Immunohistochemical analyses of colorectal cancer tissues (74 specimens) were performed with a newly established mouse monoclonal antibody, YB-3 specifically recognizing H disaccharide (Fucalpha1,2Galbeta) structures, and anti-A, anti-B, YB-2, and anti-sialyl Lewis X (SLX) antibodies, together with the analyses of glycosyltransferases involved in the synthesis of ABH antigens in the same tissues.

RESULTS:

The YB-3 antibody enabled us to detect colorectal tumors, particularly tumors in the distal large intestine and the rectum, with high sensitivity (74.3%) and specificity (100%). From immunohistochemical and enzymatic analyses of colorectal tissues, we found that once alpha1,2fucosylation had proceeded in tumor tissues, blood group A or B antigen was also synthesized in approximately half of the tissues of A or B blood type, but not in their normal tissues. A correlation of survival rate with immunostaining of tissues was found only by YB-3 antibody and not by anti-A, anti-B, or anti-SLX antibody.

CONCLUSIONS:

As a predictor of postoperative prognosis of patients with colorectal cancer, immunodetection of alpha1,2fucosylated antigens with the YB-3 antibody seemed to be superior to blood groups A, B, or SLX antigen in colorectal tumor tissues.

• Authors: Kaori Tsuboi, Takayuki Asao, Munenori Ide, Shinji Hashimoto, Kasumi Noguchi, Yoshihiko Kominato, Abby R Saniabadi, Hiroyuki Kuwano, Shin Yazawa
• Journal: Annals of surgical oncology (Ann Surg Oncol) Vol. 14 Issue 6 Pg. 1880-9 (Jun 2007) ISSN: 1068-9265 [Print] United States
• PMID: 17375356 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ABO Blood-Group System
• Antibodies, Monoclonal
• Antigens, Neoplasm
• Biomarkers, Tumor
• Disaccharides
• H-2 Antigens
• Lewis Blood Group Antigens
• Lewis X Antigen
• Oligosaccharides
• Sialyl Lewis X Antigen
• fucosyl-1-2-galactose
• Glycosyltransferases
• Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase

Topics

• ABO Blood-Group System (analysis)
• Antibodies, Monoclonal
• Antigens, Neoplasm (analysis)
• Biomarkers, Tumor (analysis)
• Colonic Neoplasms (pathology, surgery)
• Disaccharides (analysis)
• Female
• Forecasting
• Fucosyl Galactose alpha-N-Acetylgalactosaminyltransferase (analysis)
• Glycosyltransferases (analysis)
• H-2 Antigens
• Humans
• Lewis Blood Group Antigens (analysis)
• Lewis X Antigen (analysis)
• Male
• Oligosaccharides (analysis)
• Prognosis
• Rectal Neoplasms (pathology, surgery)
• Sialyl Lewis X Antigen