Induction of immunological antitumor effects by adenovirus-mediated gene transfer of B7-1 in a murine squamous cell carcinoma cell line.

Abstract

OBJECTIVE:

To evaluate the antitumor immune effects of B7-1 gene expression mediated by adenoviral vectors against squamous cell carcinoma. Transfection of the costimulatory molecule B7-1 gene into certain murine tumors increases antitumor immunity and suppresses tumor growth.

DESIGN:

In vitro and in vivo study.

INTERVENTIONS:

A murine squamous cell carcinoma cell line, KLN205, was infected with adenoviral vectors carrying either B7-1 (AdB7) or LacZ (AdCL) genes. Infected cells were injected subcutaneously into the flanks of DBA/2 mice.

MAIN OUTCOME MEASURES:

The growth of tumors infected with adenviral vectors was measured.

RESULTS:

AdB7-infected cells grew significantly slower than AdCL-infected cells in vivo, while there was no significant difference in the growth rates between the 2 groups in vitro. Moreover, significant growth suppression of rechallenged noninfected parental cells was observed in the mice immunized with AdB7-infected cells but not in those immunized with AdCL-infected cells.

CONCLUSION:

These results suggest that the B7-1 gene has therapeutic potential for immunotherapy against head and neck squamous cell carcinoma.

• Authors: Yasuhiko Hoshitani, Haruhiko Ishida, Naoki Otsuki, Toshiro Shirakawa, Akinobu Gotoh, Ken-Ichi Nibu
• Journal: Archives of otolaryngology--head & neck surgery (Arch Otolaryngol Head Neck Surg) Vol. 133 Issue 3 Pg. 270-5 (Mar 2007) ISSN: 0886-4470 [Print] United States
• PMID: 17372085 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• B7-1 Antigen

Topics

• Adenoviridae (genetics)
• Animals
• B7-1 Antigen (administration & dosage, genetics, immunology)
• Carcinoma, Squamous Cell (drug therapy, immunology)
• Cell Line, Tumor
• Disease Models, Animal
• Gene Expression
• Genetic Vectors
• Head and Neck Neoplasms (drug therapy, immunology)
• Immunotherapy, Active (methods)
• Male
• Mice
• Mice, Inbred DBA
• Transfection